A Role for the ESCRT System in Cell Division in Archaea

被引：259

作者：

Samson, Rachel Y. ^{[1
]}

Obita, Takayuki ^{[2
]}

Freund, Stefan M. ^{[3
]}

Williams, Roger L. ^{[2
]}

Bell, Stephen D. ^{[1
]}

机构：

[1] MRC, Canc Cell Unit, Cambridge CB2 0XZ, England

[2] MRC, Mol Biol Lab, Cambridge CB2 0QH, England

[3] MRC, Ctr Prot Engn, Cambridge CB2 0QH, England

来源：

SCIENCE | 2008年 / 322卷 / 5908期

基金：

英国医学研究理事会; 英国惠康基金;

关键词：

D O I：

10.1126/science.1165322

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Archaea are prokaryotic organisms that lack endomembrane structures. However, a number of hyperthermophilic members of the Kingdom Crenarchaea, including members of the Sulfolobus genus, encode homologs of the eukaryotic endosomal sorting system components Vps4 and ESCRT- III ( endosomal sorting complex required for transport- III). We found that Sulfolobus ESCRT- III and Vps4 homologs underwent regulation of their expression during the cell cycle. The proteins interacted and we established the structural basis of this interaction. Furthermore, these proteins specifically localized to the mid- cell during cell division. Overexpression of a catalytically inactive mutant Vps4 in Sulfolobus resulted in the accumulation of enlarged cells, indicative of failed cell division. Thus, the archaeal ESCRT system plays a key role in cell division.

引用

页码：1710 / 1713

页数：4