High expression of putative aquaporin genes in cells with transporting and nutritive functions during seed development in Norway spruce (Picea abies)

被引:13
作者
Hakman, I [1 ]
Oliviusson, P
机构
[1] Kalmar Univ, Dept Biol & Environm Sci, S-39182 Kalmar, Sweden
[2] Univ Stockholm, Dept Bot, S-10691 Stockholm, Sweden
关键词
aquaporin; conifer; in situ hybridization; seed; tonoplast intrinsic protein (TIP);
D O I
10.1093/jexbot/53.369.639
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Aquaporins mediate the bidirectional passage of water over membranes and are present in tonoplasts (TIPs) and in plasma membranes (PIPs) of plant cells. Knowing their expression in different tissues is valuable when assessing their contribution to plant water relations. A TIP-gene has been cloned from developing female gametophytes of Picea abies, a conifer displaying an embryology different from the angiosperms. Probes were made from conserved regions of the TIP gene and used for in situ hybridization to examine the gene expression pattern in developing female reproductive structures. Early during development high transcript expression was found in the spongy tissue encasing the developing female gametophyte, in cells of the future seed coat of young ovules and in vascular tissue of the ovuliferous scale. At later stages a strong signal was seen in archegonia jacket cells surrounding egg cells and, still later, at the time of storage protein accumulation, in storage parenchyma cells of the gametophyte as well. These aquaporin-homologues probably participate in regulating water balance in the cells although they could also be permeable to other molecules than water.
引用
收藏
页码:639 / 649
页数:11
相关论文
共 48 条
  • [21] Water transport activity of the plasma membrane aquaporin PM28A is regulated by phosphorylation
    Johansson, I
    Karlsson, M
    Shukla, VK
    Chrispeels, MJ
    Larsson, C
    Kjellbom, P
    [J]. PLANT CELL, 1998, 10 (03) : 451 - 459
  • [22] The major integral proteins of spinach leaf plasma membranes are putative aquaporins and are phosphorylated in response to Ca2+ and apoplastic water potential
    Johansson, I
    Larsson, C
    Ek, B
    Kjellbom, P
    [J]. PLANT CELL, 1996, 8 (07) : 1181 - 1191
  • [23] AN ABUNDANT, HIGHLY CONSERVED TONOPLAST PROTEIN IN SEEDS
    JOHNSON, KD
    HERMAN, EM
    CHRISPEELS, MJ
    [J]. PLANT PHYSIOLOGY, 1989, 91 (03) : 1006 - 1013
  • [24] TONOPLAST-BOUND PROTEIN-KINASE PHOSPHORYLATES TONOPLAST INTRINSIC PROTEIN
    JOHNSON, KD
    CHRISPEELS, MJ
    [J]. PLANT PHYSIOLOGY, 1992, 100 (04) : 1787 - 1795
  • [25] A water channel of the nematode C-elegans and its implications for channel selectivity of MIP proteins
    Kuwahara, M
    Ishibashi, K
    Gu, Y
    Terada, Y
    Kohara, Y
    Marumo, F
    Sasaki, S
    [J]. AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY, 1998, 275 (06): : C1459 - C1464
  • [26] Oligomerization state of water channels and glycerol facilitators -: Involvement of loop E
    Lagrée, V
    Froger, A
    Deschamps, S
    Pellerin, I
    Delamarche, C
    Bonnec, G
    Gouranton, J
    Thomas, D
    Hubert, JF
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (51) : 33949 - 33953
  • [27] Switch from an aquaporin to a glycerol channel by two amino acids substitution
    Lagrée, V
    Froger, A
    Deschamps, S
    Hubert, JF
    Delamarche, C
    Bonnec, G
    Thomas, D
    Gouranton, J
    Pellerin, I
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (11) : 6817 - 6819
  • [28] THE EXPRESSION PATTERN OF THE TONOPLAST INTRINSIC PROTEIN GAMMA-TIP IN ARABIDOPSIS-THALIANA IS CORRELATED WITH CELL ENLARGEMENT
    LUDEVID, D
    HOFTE, H
    HIMELBLAU, E
    CHRISPEELS, MJ
    [J]. PLANT PHYSIOLOGY, 1992, 100 (04) : 1633 - 1639
  • [29] Desiccation- and abscisic acid-responsive genes encoding major intrinsic proteins (MIPs) from the resurrection plant Craterostigma plantagineum
    Mariaux, JB
    Bockel, C
    Salamini, F
    Bartels, D
    [J]. PLANT MOLECULAR BIOLOGY, 1998, 38 (06) : 1089 - 1099
  • [30] Aquaporins and water permeability of plant membranes
    Maurel, C
    [J]. ANNUAL REVIEW OF PLANT PHYSIOLOGY AND PLANT MOLECULAR BIOLOGY, 1997, 48 : 399 - 429