Standardized Methods and Quality Control Limits for Agar and Broth Microdilution Susceptibility Testing of Mycoplasma pneumoniae, Mycoplasma hominis, and Ureaplasma urealyticum

被引：71

作者：

Waites, Ken B. ^{[1
]}

Duffy, Lynn B. ^{[1
]}

Bebear, Cecile M. ^{[2
]}

Matlow, Anne ^{[3
]}

Talkington, Deborah F. ^{[4
]}

Kenny, George E. ^{[5
]}

Totten, Patricia A. ^{[5
]}

Bade, Donald J. ^{[6
]}

Zheng, Xiaotian ^{[7
]}

Davidson, Maureen K. ^{[8
]}

Shortridge, Virginia D. ^{[9
]}

Watts, Jeffrey L. ^{[10
]}

Brown, Steven D. ^{[11
]}

机构：

[1] Univ Alabama Birmingham, Dept Pathol, Birmingham, AL 35294 USA

[2] Univ Bordeaux, Unite Contrat Mycoplasmal & Chlamydial Infect Hum, Bordeaux, France

[3] Hosp Sick Children, Toronto, ON M5G 1X8, Canada

[4] Ctr Dis Control & Prevent, Atlanta, GA USA

[5] Univ Washington, Sch Publ Hlth, Dept Med, Seattle, WA 98195 USA

[6] Microbial Res Inc, Ft Collins, CO USA

[7] Childrens Mem Hosp, Chicago, IL 60614 USA

[8] FDA Ctr Vet Med, Laurel, MD USA

[9] BioMerieux Inc, Hazewood, MO USA

[10] Pfizer Anim Hlth, Kalamazoo, MI USA

[11] Inst Clin Microbiol, Wilsonville, OR USA

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2012年 / 50卷 / 11期

关键词：

D O I：

10.1128/JCM.01439-12

中图分类号：

Q93 [微生物学];

学科分类号：

071005 [微生物学];

摘要：

An international multilaboratory collaborative study was conducted to develop standard media and consensus methods for the performance and quality control of antimicrobial susceptibility testing of Mycoplasma pneumoniae, Mycoplasma hominis, and Ureaplasma urealyticum using broth microdilution and agar dilution techniques. A reference strain from the American Type Culture Collection was designated for each species, which was to be used for quality control purposes. Repeat testing of replicate samples of each reference strain by participating laboratories utilizing both methods and different lots of media enabled a 3-to4-dilution MIC range to be established for drugs in several different classes, including tetracyclines, macrolides, ketolides, lincosamides, and fluoroquinolones. This represents the first multilaboratory collaboration to standardize susceptibility testing methods and to designate quality control parameters to ensure accurate and reliable assay results for mycoplasmas and ureaplasmas that infect humans.

引用

页码：3542 / 3547

页数：6

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