Aberrant termination triggers nonsense-mediated mRNA decay

被引：60

作者：

Amrani, N ^{[1
]}

Dong, S ^{[1
]}

He, F ^{[1
]}

Ganesan, R ^{[1
]}

Ghosh, S ^{[1
]}

Kervestin, S ^{[1
]}

Li, C ^{[1
]}

Mangus, DA ^{[1
]}

Spatrick, P ^{[1
]}

Jacobson, A ^{[1
]}

机构：

[1] Univ Massachusetts, Sch Med, Dept Mol Genet & Microbiol, Worcester, MA 01655 USA

来源：

BIOCHEMICAL SOCIETY TRANSACTIONS | 2006年 / 34卷

关键词：

faux untransrated region; mRNA decay; translation termination; yeast;

D O I：

10.1042/BST0340039

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

NMD (nonsense-mediated mRNA decay) is a cellular quality-control mechanism in which an otherwise stable mRNA is destabilized by the presence of a premature termination codon. We have defined the set of endogenous NMD substrates, demonstrated that they are available for NMD at every round of translation, and showed that premature termination and normal termination are not equivalent biochemical events. Premature termination is aberrant, and its NMD-stimulating defects can be reversed by the presence of tethered poly(A)-binding protein (Pab1p) or tethered eRF3 (eukaryotic release factor 3) (Sup35p). Thus NMD appears to be triggered by a ribosome's failure to terminate adjacent to a properly configured 3'-UTR (untranslated region), an event that may promote binding of the UPF/NMD factors to stimulate mRNA decapping.

引用

页码：39 / 42

页数：4

共 48 条

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