Distinct classes of yeast promoters revealed by differential TAF recruitment

被引：158

作者：

Li, XY ^{[1
]}

Bhaumik, SR ^{[1
]}

Green, MR ^{[1
]}

机构：

[1] Univ Massachusetts, Sch Med, Program Mol Med, Howard Hughes Med Inst, Worcester, MA 01605 USA

来源：

SCIENCE | 2000年 / 288卷 / 5469期

关键词：

D O I：

10.1126/science.288.5469.1242

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The transcription factor TFIID contains the TATA box binding protein (TBP) and multiple TBP-associated factors (TAFs), Here, the association of TFIID components with promoters that either are dependent on multiple TAFs (TAF(dep)) or have no apparent TAF requirement (TAF(ind)) is analyzed in yeast. At TAF(dep) promoters, TAFs are present at Levels comparable to that of TBP, whereas at TAF(ind) promoters, TAFs are present at Levels that approximate background. After inactivation of several general transcription factors, including TBP, TAFs are still recruited by activators to TAF(dep) promoters. The results reveal two classes of promoters: at TAF(ind) promoters, TBP is recruited in the apparent absence of TAFs, whereas at TAF(dep) promoters, TAFs are co-recruited with TBP in a manner consistent with direct activator-TAF interactions.

引用

页码：1242 / 1244

页数：3

共 18 条

[1] Yeast TAF(II)90 is required for cell-cycle progression through G(2)/M but not for general transcription activation [J].

Apone, LM ;

Virbasius, CMA ;

Reese, JC ;

Green, MR .

GENES & DEVELOPMENT, 1996, 10 (18) :2368-2380

[2] Broad, but not universal, transcriptional requirement for yTAFII17, a histone H3-like TAFII present in TFIID and SAGA [J].

Apone, LM ;

Virbasius, CA ;

Holstege, FCP ;

Wang, J ;

Young, RA ;

Green, MR .

MOLECULAR CELL, 1998, 2 (05) :653-661

[3] Biochemistry and structural biology of transcription factor IID (TFIID) [J].

Burley, SK ;

Roeder, RG .

ANNUAL REVIEW OF BIOCHEMISTRY, 1996, 65 :769-799

[4] THE TATA-BINDING PROTEIN IS REQUIRED FOR TRANSCRIPTION BY ALL 3 NUCLEAR-RNA POLYMERASES IN YEAST-CELLS [J].

CORMACK, BP ;

STRUHL, K .

CELL, 1992, 69 (04) :685-696

[5] TBP-associated factors (TAFIIs):: multiple, selective transcriptional mediators in common complexes [J].

Green, MR .

TRENDS IN BIOCHEMICAL SCIENCES, 2000, 25 (02) :59-63

[6] Dissecting the regulatory circuitry of a eukaryotic genome [J].

Holstege, FCP ;

Jennings, EG ;

Wyrick, JJ ;

Lee, TI ;

Hengartner, CJ ;

Green, MR ;

Golub, TR ;

Lander, ES ;

Young, RA .

CELL, 1998, 95 (05) :717-728

[7] TAF-containing and TAF-independent forms of transcriptionally active TBP in vivo [J].

Kuras, L ;

Kosa, P ;

Mencia, M ;

Struhl, K .

SCIENCE, 2000, 288 (5469) :1244-1248

[8] Binding of TBP to promoters in vivo is stimulated by activators and requires Pol II holoenzyme [J].

Kuras, L ;

Struhl, K .

NATURE, 1999, 399 (6736) :609-613

[9]

LEE TI, IN PRESS NATURE

[10] Enhancement of TBP binding by activators and general transcription factors [J].

Li, XY ;

Virbasius, A ;

Zhu, XC ;

Green, MR .

NATURE, 1999, 399 (6736) :605-609

← 1 2 →