Distinct classes of yeast promoters revealed by differential TAF recruitment

被引:158
作者
Li, XY [1 ]
Bhaumik, SR [1 ]
Green, MR [1 ]
机构
[1] Univ Massachusetts, Sch Med, Program Mol Med, Howard Hughes Med Inst, Worcester, MA 01605 USA
关键词
D O I
10.1126/science.288.5469.1242
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The transcription factor TFIID contains the TATA box binding protein (TBP) and multiple TBP-associated factors (TAFs), Here, the association of TFIID components with promoters that either are dependent on multiple TAFs (TAF(dep)) or have no apparent TAF requirement (TAF(ind)) is analyzed in yeast. At TAF(dep) promoters, TAFs are present at Levels comparable to that of TBP, whereas at TAF(ind) promoters, TAFs are present at Levels that approximate background. After inactivation of several general transcription factors, including TBP, TAFs are still recruited by activators to TAF(dep) promoters. The results reveal two classes of promoters: at TAF(ind) promoters, TBP is recruited in the apparent absence of TAFs, whereas at TAF(dep) promoters, TAFs are co-recruited with TBP in a manner consistent with direct activator-TAF interactions.
引用
收藏
页码:1242 / 1244
页数:3
相关论文
共 18 条
[11]   Histone-like TAFs are essential for transcription in vivo [J].
Michel, B ;
Komarnitsky, P ;
Buratowski, S .
MOLECULAR CELL, 1998, 2 (05) :663-673
[12]   Yeast homologues of higher eukaryotic TFIID subunits [J].
Moqtaderi, Z ;
Yale, JD ;
Struhl, K ;
Buratowski, S .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (25) :14654-14658
[13]   TBP-associated factors are not generally required for transcriptional activation in yeast [J].
Moqtaderi, Z ;
Bai, Y ;
Poon, D ;
Weil, PA ;
Struhl, K .
NATURE, 1996, 383 (6596) :188-191
[14]   EUKARYOTIC RNA-POLYMERASE CONDITIONAL MUTANT THAT RAPIDLY CEASES MESSENGER-RNA SYNTHESIS [J].
NONET, M ;
SCAFE, C ;
SEXTON, J ;
YOUNG, R .
MOLECULAR AND CELLULAR BIOLOGY, 1987, 7 (05) :1602-1611
[15]   Yeast TAF(II)145 functions as a core promoter selectivity factor, not a general coactivator [J].
Shen, WC ;
Green, MR .
CELL, 1997, 90 (04) :615-624
[16]   GENERAL REQUIREMENT FOR RNA-POLYMERASE-II HOLOENZYMES IN-VIVO [J].
THOMPSON, CM ;
YOUNG, RA .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (10) :4587-4590
[17]   TAFs mediate transcriptional activation and promoter selectivity [J].
Verrijzer, CP ;
Tjian, R .
TRENDS IN BIOCHEMICAL SCIENCES, 1996, 21 (09) :338-342
[18]   Transcription activation in cells lacking TAF(II)s [J].
Walker, SS ;
Reese, JC ;
Apone, LM ;
Green, MR .
NATURE, 1996, 383 (6596) :185-188