Application of microarray technology for microbial diagnosis in stem cell cultures: a review

被引：2

作者：

Cobo, F. ^{[1
]}

Concha, A. ^{[1
]}

机构：

[1] Stem Cell Bank Andalucia Spanish Cent Node, Granada, Spain

来源：

CYTOTHERAPY | 2007年 / 9卷 / 01期

关键词：

cell cultures; cytotherapy; DNA chips; DNA microarrays; microbial diagnosis; stem cell research;

D O I：

10.1080/14653240601113205

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

Stem cell lines used for cell therapy and regenerative medicine programs could he contaminated by several types of microorganism, such as bactria, fungi, yeasts, viruses and prion particles. The presence of these pathogens makes the stem cell cultures unsuitable for transplant in humans. At the moment, tests for detecting these kinds of pathogens are carried out by means of standardized diagnosis procedures, in order to avoid the possibility of transmitting infectious diseases to the recipients of stem cell products. Some of the methods that can be included in a microbiologic control program are culture-based methods for sterility assessment, molecular techniques (PCR, RT-PCR), Ag detection and electron microscopy. However, new technologies on DNA microarrays and protein arrays could also be applied for microbial diagnosis in stem cell lines in order to improve the microorganism detection. In this review, we summarize the main features concerning microarray methodology, the advantages and disadvantages regarding microbial diagnosis for stem cell cultures and possible future application in stem cell research centers in the microbiology field.

引用

页码：53 / 59

页数：7

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