Development of a semiquantitative degenerate real-time PCR-based assay for estimation of numbers of butyryl-coenzyme A (CoA) CoA transferase genes in complex bacterial samples

被引：164

作者：

Louis, Petra ^{[1
]}

Flint, Harry J. ^{[1
]}

机构：

[1] Rowett Res Inst, Microbial Ecol Grp, Gut Hlth Div, Bucksburn AB21 9SB, Aberdeen, Scotland

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2007年 / 73卷 / 06期

关键词：

D O I：

10.1128/AEM.02561-06

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We describe a new degenerate real-time PCR approach to simultaneously quantify phylogenetically different butyrate-producing bacteria based on the detection of butyryll-coenzyme A (CoA) CoA transferase genes. This pathway is present in numerically important groups of butyrate producers within the human colon, and thus this assay estimates the butyrate-producing ability of the microbiota.

引用

页码：2009 / 2012

页数：4

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