Quantitative genetic analysis in Saccharomyces cerevisiae using epistatic miniarray profiles (E-MAPs) and its application to chromatin functions

被引：65

作者：

Schuldiner, M. ^{[1
]}

Collins, S. R. ^{[1
]}

Weissman, J. S. ^{[1
]}

Krogan, N. J. ^{[1
]}

机构：

[1] Univ Calif San Francisco, Dept Cellular & Mol Pharmacol, Calif Inst Quantitat Biomed Res, San Francisco, CA 94143 USA

来源：

METHODS | 2006年 / 40卷 / 04期

关键词：

chromatin; quantitative; genetic interactions; epistasis; E-MAP; Saccharomyces cerevisiae; DAmP;

D O I：

10.1016/j.ymeth.2006.07.034

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The use of the budding yeast Saccharomyces cerevisiae as a simple eukaryotic model system for the study of chromatin assembly and regulation has allowed rapid discovery of genes that influence this complex process. The functions of many of the proteins encoded by these genes have not yet been fully characterized. Here, we describe a high-throughput methodology that can be used to illuminate gene function and discuss its application to a set of genes involved in the creation, maintenance and remodeling of chromatin structure. Our technique, termed E-MAPs, involves the generation of quantitative genetic interaction maps that reveal the function and organization of cellular proteins and networks. (c) 2006 Elsevier Inc. All rights reserved.

引用

页码：344 / 352

页数：9

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