Intragenic recombinants of Plasmodium falciparum identified by in situ polymerase chain reaction

被引:9
作者
Ranford-Cartwright, L [1 ]
Walliker, D [1 ]
机构
[1] Univ Edinburgh, Inst Cell Anim & Populat Biol, Edinburgh EH9 3JT, Midlothian, Scotland
基金
英国医学研究理事会;
关键词
Plasmodium falciparum; in situ polymerase chain reaction; intragenic recombination; merozoite surface protein;
D O I
10.1016/S0166-6851(99)00069-9
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We report an in situ PCR technique for visualising amplified DNA of blood forms of Plasmodium falciparum on microscope slides by fluorescence microscopy. The method is used to assess the changes in frequency of different alleles of the MSPI gene in cultures of the progeny of a cross. We show that parasites with a recombinant form of this protein possess an initial growth advantage before declining in numbers over the long-term. (C) 1999 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:13 / 20
页数:8
相关论文
共 23 条
[1]   IDENTIFICATION OF PARASITE PROTEINS IN A MEMBRANE PREPARATION ENRICHED FOR THE SURFACE-MEMBRANE OF ERYTHROCYTES INFECTED WITH PLASMODIUM-KNOWLESI [J].
ALEY, SB ;
BARNWELL, JW ;
DANIEL, W ;
HOWARD, RJ .
MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1984, 12 (01) :69-84
[2]   DETECTION OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 PROVIRUS IN MONONUCLEAR-CELLS BY INSITU POLYMERASE CHAIN-REACTION [J].
BAGASRA, O ;
HAUPTMAN, SP ;
LISCHNER, HW ;
SACHS, M ;
POMERANTZ, RJ .
NEW ENGLAND JOURNAL OF MEDICINE, 1992, 326 (21) :1385-1391
[3]  
CHUI KP, 1992, J HISTOCHEM CYTOCHEM, V40, P333
[4]   PLASMODIUM-FALCIPARUM - INTRAGENIC RECOMBINATION AND NONRANDOM ASSOCIATIONS BETWEEN POLYMORPHIC DOMAINS OF THE PRECURSOR TO THE MAJOR MEROZOITE SURFACE-ANTIGENS [J].
CONWAY, DJ ;
ROSARIO, V ;
ODUOLA, AMJ ;
SALAKO, LA ;
GREENWOOD, BM ;
MCBRIDE, JS .
EXPERIMENTAL PARASITOLOGY, 1991, 73 (04) :469-480
[5]   POPULATION-GENETICS OF PLASMODIUM-FALCIPARUM WITHIN A MALARIA HYPERENDEMIC AREA [J].
CONWAY, DJ ;
MCBRIDE, JS .
PARASITOLOGY, 1991, 103 :7-16
[6]   PROTEIN VARIATION IN CLONES OF PLASMODIUM-FALCIPARUM DETECTED BY 2 DIMENSIONAL ELECTROPHORESIS [J].
FENTON, B ;
WALKER, A ;
WALLIKER, D .
MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1985, 16 (02) :173-183
[7]  
GOSDEN J, 1993, BIOTECHNIQUES, V15, P78
[8]   AMPLIFICATION AND DETECTION OF LENTIVIRAL DNA INSIDE CELLS [J].
HAASE, AT ;
RETZEL, EF ;
STASKUS, KA .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (13) :4971-4975
[9]   CULTURE OF HUMAN MALARIA PARASITES PLASMODIUM-FALCIPARUM [J].
HAYNES, JD ;
DIGGS, CL ;
HINES, FA ;
DESJARDINS, RE .
NATURE, 1976, 263 (5580) :767-769
[10]   SEQUENCE VARIATION IN THE TRIPEPTIDE REPEATS AND T-CELL EPITOPES IN P190 (MSA-1) OF PLASMODIUM-FALCIPARUM FROM FIELD ISOLATES [J].
JONGWUTIWES, S ;
TANABE, K ;
NAKAZAWA, S ;
YANAGI, T ;
KANBARA, H .
MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1992, 51 (01) :81-90