Intragenic recombinants of Plasmodium falciparum identified by in situ polymerase chain reaction

被引：9

作者：

Ranford-Cartwright, L ^{[1
]}

Walliker, D ^{[1
]}

机构：

[1] Univ Edinburgh, Inst Cell Anim & Populat Biol, Edinburgh EH9 3JT, Midlothian, Scotland

来源：

MOLECULAR AND BIOCHEMICAL PARASITOLOGY | 1999年 / 102卷 / 01期

基金：

英国医学研究理事会;

关键词：

Plasmodium falciparum; in situ polymerase chain reaction; intragenic recombination; merozoite surface protein;

D O I：

10.1016/S0166-6851(99)00069-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We report an in situ PCR technique for visualising amplified DNA of blood forms of Plasmodium falciparum on microscope slides by fluorescence microscopy. The method is used to assess the changes in frequency of different alleles of the MSPI gene in cultures of the progeny of a cross. We show that parasites with a recombinant form of this protein possess an initial growth advantage before declining in numbers over the long-term. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：13 / 20

页数：8

共 23 条

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