Determination of γ-hydroxybutyric acid in clinical samples using capillary electrophoresis with contactless conductivity detection

被引：28

作者：

Gong, Xiao Yang ^{[1
]}

Kuban, Pavel ^{[2
]}

Scholer, Andre ^{[3
]}

Hauser, Peter C. ^{[1
]}

机构：

[1] Univ Basel, Dept Chem, CH-4056 Basel, Switzerland

[2] Acad Sci Czech Republ, Inst Analyt Chem, CZ-60200 Brno, Czech Republic

[3] Univ Hosp, Clin Chem Lab, CH-4031 Basel, Switzerland

来源：

JOURNAL OF CHROMATOGRAPHY A | 2008年 / 1213卷 / 01期

基金：

瑞士国家科学基金会;

关键词：

gamma-Hydroxybutyric acid (GHB); Vancomycin; Capillary electrophoresis; Contactless conductivity detection; Urine; Serum;

D O I：

10.1016/j.chroma.2008.10.015

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method for the determination of gamma-hydroxybutyric acid (GHB) in urine and serum samples with capillary electrophoresis using capacitively coupled contactless conductivity detection (CE-(CD)-D-4) was developed. The optimized separation buffer consisting of 20 mM of arginine, 10 mM of maleic acid and 30 mu M of cetyltrimethylammonium bromide (CTAB) contained 5 mM vancomycin to facilitate the separation of gamma-hydroxybutyric acid from beta-hydroxybutyric acid (BHB), which is also present in clinical samples. The detection limits in the clinical samples were found to be about 2 mu g/ml, which is well below the required sensitivity for the recognition of overdosage and adequate for the determination of endogenous concentrations in urine. The determination of GHB in both types of samples was carried out directly after a fourfold dilution without requiring any derivatization or extraction procedures. (c) 2008 Elsevier B.V. All rights reserved.

引用

页码：100 / 104

页数：5

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