Production of bioactive triterpenes by Eriobotrya japonica calli

被引:305
作者
Taniguchi, S
Imayoshi, Y
Kobayashi, E
Takamatsu, Y
Ito, H
Hatano, T
Sakagami, H
Tokuda, H
Nishino, H
Sugita, D
Shimura, S
Yoshida, T [1 ]
机构
[1] Okayama Univ, Fac Pharmaceut Sci, Okayama 7008530, Japan
[2] Meikai Univ, Sch Dent, Dept Dent Pharmacol, Sakado, Saitama 3500283, Japan
[3] Kyoto Prefectural Univ Med, Kamigyo Ku, Kyoto 6020841, Japan
[4] Lotte Cent Lab, Numakage, Saitama 3368601, Japan
关键词
Eriobotrya japonica; Rosaceae; callus tissue culture; triterpene; Epstein-Barr virus; two-stage carcinogenesis;
D O I
10.1016/S0031-9422(01)00455-1
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Callus tissue cultures induced from an axenic leaf of Eriobotrya japonica (Rosaceae) produced triterpenes in large amounts (ca. 50 mg/g dry wt). Nine triterpenes were characterized as ursolic acid, oleanolic acid, 2alpha-hydoxyursolic acid, maslinic acid, tormentic acid, 2alpha, 19alpha-dihydroxy-3-oxo-urs-12-en-28-oic acid, hyptadienic acid and a mixture of 3-O-cis-p-coumaroyltormentic acid and 3-O-trans-p-coumaroyltormentic acid. The triterpene composition in the callus tissues was noticeably different from that in intact leaves. The contents of tormentic acid with antidiabetic action, and 2alpha, 19alpha-dihydroxy-3-oxo-urs-12-en-28-oic acid with anti-HIV activity, were much larger than those in the intact leaves. All of the triterpenes isolated from the callus tissues showed an inhibitory effect comparable to (-)-epigallocatechin gallate (EGCG) of green tea on the activation of Epstein-Barr virus early antigen (EBV-EA) induced by 12-O-tetradecanoylphorbol-13-acetate (TPA). 2alpha, 19alpha-Dihydroxy-3-oxo-urs-12-en-28-oic acid was the most potent inhibitor among them and caused a significant delay of two-stage carcinogenesis on mouse skin. (C) 2002 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:315 / 323
页数:9
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