Functional expression of plant alternative oxidase decreases antimycin A-induced reactive oxygen species production in human cells

被引：15

作者：

Matsukawa, Kazushige ^{[2
]}

Kamata, Takashi ^{[1
]}

Ito, Kikukatsu ^{[1
,2
]}

机构：

[1] Iwate Univ, Fac Agr, Cryobiofrontier Res Ctr, Morioka, Iwate 0208550, Japan

[2] Iwate Univ, United Grad Sch Agr Sci, Morioka, Iwate 0208550, Japan

来源：

FEBS LETTERS | 2009年 / 583卷 / 01期

基金：

日本学术振兴会;

关键词：

Alternative oxidase; Antimycin A; HeLa cell; Mitochondria; Reactive oxygen species; Symplocarpus renifolius; MITOCHONDRIAL DYSFUNCTION; RESPIRATORY-CHAIN; THERMOGENESIS; SUPEROXIDE; INHIBITION; RESISTANCE; PATHWAY; BCL-2;

D O I：

10.1016/j.febslet.2008.11.040

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Alternative oxidase (AOX) plays a pivotal role in cyanide-resistance respiration in the mitochondria of plants, fungi and some protists. Here we show that AOX from thermogenic skunk cabbage successfully conferred cyanide resistance to human cells. In galactose medium, HeLa cells with mitochondria-targeted AOX proteins were found to have significantly less reactive oxygen species production in response to antimycin-A exposure, a specific inhibitor of respiratory complex III. These results suggest that skunk cabbage AOX can be used to create an alternative respiration pathway, which might be important for therapy against various mitochondrial diseases. (C) 2008 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.

引用

页码：148 / 152

页数：5

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