Differential regulation of chloroplast gene expression in Chlamydomonas reinhardtii during photoacclimation: Light stress transiently suppresses synthesis of the Rubisco LSU protein while enhancing synthesis of the PS II D1 protein

被引:68
作者
Shapira, M
Lers, A
Heifetz, PB
Irihimovitz, V
Osmond, CB
Gillham, NW
Boynton, JE
机构
[1] DUKE UNIV,DEPT BOT,DCMB GRP,DURHAM,NC 27708
[2] DUKE UNIV,DEPT ZOOL,DURHAM,NC 27708
关键词
chloroplast protein synthesis; D1; LSU; photoinhibition; translational regulation;
D O I
10.1023/A:1005814800641
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Transfer of Chlamydomonas reinhardtii cells grown photoautotrophically in low light to higher light intensities has a dramatic transient effect on the differential expression of the two major chloroplast encoded photosynthetic proteins. Synthesis of the D1 protein of Photosystem II increases more than 10-fold during the first six hours in high light (HL), whereas synthesis of the large subunit (LSU) of Rubisco drops dramatically within 15 min and only gradually resumes at about 6 h. Synthesis of the chloroplast-encoded ATP synthase beta subunit, the nuclear-encoded Rubisco small subunit and the nuclear-encoded beta-tubulin is not noticeably affected. Up regulation of psbA mRNA translation accounts for a substantial fraction of the increased Di synthesis, since accumulation of psbA mRNA increases 4.2- and 6.3-fold less than D1 synthesis at 6 and 18 h in HL. Down-regulation of LSU synthesis is not correlated with a reduction in the steady-state level of the rbcL transcript. Primer extension mapping of the 5' ends of the rbcL mRNAs reveals transcripts with start points located at -93 and -168 relative to the first translated ATG. Transfer of low light (LL)-grown cells to HL temporarily decreases the ratio of the -93 to -168 transcripts, but this ratio normalizes after 6 h in HL, coincident with the recovery in the synthesis of LSU. These several distinct effects of temporary Light stress were correlated with a rapid, sustained increase in the reduction state of Q(A), a transient decline in photosynthetic efficiency, a less rapid drop in total chlorophyll content and a delay in cell division.
引用
收藏
页码:1001 / 1011
页数:11
相关论文
共 40 条
[32]  
Prasil O., 1992, PHOTOSYSTEMS STRUCTU, V11, P295, DOI DOI 10.1016/B978-0-444-89440-3.50017-5
[33]  
REINBOTHE S, 1993, J BIOL CHEM, V268, P10606
[34]   A METHYL JASMONATE-INDUCED SHIFT IN THE LENGTH OF THE 5' UNTRANSLATED REGION IMPAIRS TRANSLATION OF THE PLASTID RBCL TRANSCRIPT IN BARLEY [J].
REINBOTHE, S ;
REINBOTHE, C ;
HEINTZEN, C ;
SEIDENBECHER, C ;
PARTHIER, B .
EMBO JOURNAL, 1993, 12 (04) :1505-1512
[35]  
RINTAMAKI E, 1994, BRID PLANTA, V193, P520
[36]  
SAMBROK J, 1989, MOL CLONING
[37]   PROCESSING OF THE PRECURSOR TO A CHLOROPLAST RIBOSOMAL-PROTEIN MADE IN THE CYTOSOL OCCURS IN 2 STEPS, ONE OF WHICH DEPENDS ON A PROTEIN MADE IN THE CHLOROPLAST [J].
SCHMIDT, RJ ;
GILLHAM, NW ;
BOYNTON, JE .
MOLECULAR AND CELLULAR BIOLOGY, 1985, 5 (05) :1093-1099
[38]  
SCHMIDT RJ, 1984, THESIS DUKE U
[39]   CONTINUOUS RECORDING OF PHOTOCHEMICAL AND NONPHOTOCHEMICAL CHLOROPHYLL FLUORESCENCE QUENCHING WITH A NEW TYPE OF MODULATION FLUOROMETER [J].
SCHREIBER, U ;
SCHLIWA, U ;
BILGER, W .
PHOTOSYNTHESIS RESEARCH, 1986, 10 (1-2) :51-62
[40]   TURNOVER OF THYLAKOID PHOTOSYSTEM-II PROTEINS DURING PHOTOINHIBITION OF CHLAMYDOMONAS-REINHARDTII [J].
SCHUSTER, G ;
TIMBERG, R ;
OHAD, I .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1988, 177 (02) :403-410