A 4,103 marker integrated physical and comparative map of the horse genome

被引：43

作者：

Raudsepp, T. ^{[1
]}

Gustafson-Seabury, A. ^{[1
]}

Durkin, K. ^{[1
]}

Wagner, M. L. ^{[2
]}

Goh, G. ^{[1
]}

Seabury, C. M. ^{[1
]}

Brinkmeyer-Langford, C. ^{[1
]}

Lee, E. -J. ^{[1
]}

Agarwala, R. ^{[3
]}

Stallknecht-Rice, E. ^{[3
]}

Schaeffer, A. A. ^{[3
]}

Skow, L. C. ^{[1
]}

Tozaki, T. ^{[4
]}

Yasue, H. ^{[5
]}

Penedo, M. C. T. ^{[6
]}

Lyons, L. A. ^{[7
]}

Khazanehdari, K. A. ^{[8
]}

Binns, M. M. ^{[9
]}

MacLeod, J. N. ^{[10
]}

Distl, O. ^{[11
]}

Guerin, G. ^{[12
]}

Leeb, T. ^{[13
]}

Mickelson, J. R. ^{[2
]}

Chowdhary, B. P. ^{[1
]}

机构：

[1] Texas A&M Univ, Dept Vet Integrat Biosci, College Stn, TX 77843 USA

[2] Univ Minnesota, Dept Vet Biosci, St Paul, MN 55108 USA

[3] NCBI, NIH, DHHS, Bethesda, MD USA

[4] Lab Racing Chem, Utsunomiya, Tochigi, Japan

[5] Natl Inst Agrobiol Sci, Tsukuba, Ibaraki, Japan

[6] Univ Calif Davis, Vet Genet Lab, Davis, CA 95616 USA

[7] Univ Calif Davis, Dept Populat Hlth & Reprod, Davis, CA 95616 USA

[8] Cent Vet Res Lab, Dubai, U Arab Emirates

[9] Univ London Royal Vet Coll, London, England

[10] Univ Kentucky, Dept Vet Sci, Gluck Equine Res Ctr, Lexington, KY 40546 USA

[11] Univ Vet Med Hannover, Inst Genet & Anim Breeding, Hannover, Germany

[12] INRA, Ctr Rech Jouy En Josas, F-78350 Jouy En Josas, France

[13] Univ Bern, Inst Genet, Bern, Switzerland

来源：

CYTOGENETIC AND GENOME RESEARCH | 2008年 / 122卷 / 01期

关键词：

D O I：

10.1159/000151313

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

A comprehensive second-generation whole genome radiation hybrid (RH II), cytogenetic and comparative map of the horse genome (2n = 64) has been developed using the 5000rad horse X hamster radiation hybrid panel and fluorescence in situ hybridization (FISH). The map contains 4,103 markers (3,816 RH; 1,144 FISH) assigned to all 31 pairs of autosomes and the X chromosome. The RH maps of individual chromosomes are anchored and oriented using 857 cytogenetic markers. The overall resolution of the map is one marker per 775 kilobase pairs (kb), which represents a more than five-fold improvement over the first-generation map. The RH II incorporates 920 markers shared jointly with the two recently reported meiotic maps. Consequently the two maps were aligned with the RH II maps of individual autosomes and the X chromosome. Additionally, a comparative map of the horse genome was generated by connecting 1,904 loci on the horse map with genome sequences available for eight diverse vertebrates to highlight regions of evolutionarily conserved syntenies, linkages, and chromosomal breakpoints. The integrated map thus obtained presents the most comprehensive information on the physical and comparative organization of the equine genome and will assist future assemblies of whole genome BAC fingerprint maps and the genome sequence. It will also serve as a tool to identify genes governing health, disease and performance traits in horses and assist us in understanding the evolution of the equine genome in relation to other species. Copyright (C) 2008 S. Karger AG, Basel

引用

页码：28 / 36

页数：9

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