Visualizing cells at the nanoscale

被引:149
作者
Leis, Andrew [1 ]
Rockel, Beate [1 ]
Andrees, Lars [1 ]
Baumeister, Wolfgang [1 ]
机构
[1] Max Planck Inst Biochem, Dept Mol Struct Biol, D-82152 Martinsried, Germany
关键词
X-RAY MICROSCOPY; ELECTRON TOMOGRAPHY; CRYOELECTRON TOMOGRAPHY; 3-DIMENSIONAL STRUCTURE; CORRELATIVE MICROSCOPY; SPATIAL-RESOLUTION; FROZEN; RECONSTRUCTION; CRYOMICROSCOPY; ARCHITECTURE;
D O I
10.1016/j.tibs.2008.10.011
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Cryogenic electron tomography (cryo- ET) enables the 3D visualization of biological material at a previously unseeable scale. Carefully controlled cryogenic specimen preparation avoids the artefacts that are notorious to conventional electron microscopy specimen preparation. To date, studies employing cryo- ET have mostly been restricted to isolated macromolecular assemblies, small prokaryotic cells or thin regions of eukaryotic cells owing to the limited penetration depth of electrons through ice-embedded preparations. Recent progress in cryosectioning makes it possible to acquire tomograms from many kinds of vitrified cells and tissues, The systematic and comprehensive interpretation of such tomograms will provide unprecedented insight into the molecular organization of cellular landscapes.
引用
收藏
页码:60 / 70
页数:11
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