Selenium-containing xanthine dehydrogenase from Eubacterium barkeri

被引:45
作者
Schräder, T
Rienhöfer, A
Andreesen, JR
机构
[1] Univ Halle Wittenberg, Inst Mikrobiol, D-06099 Halle, Germany
[2] Univ Gottingen, Inst Mikrobiol, D-3400 Gottingen, Germany
来源
EUROPEAN JOURNAL OF BIOCHEMISTRY | 1999年 / 264卷 / 03期
关键词
Eubacterium barkeri; molybdenum; selenium; tungsten; xanthine dehydrogenase;
D O I
10.1046/j.1432-1327.1999.00678.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A specific dehydrogenase, different from nicotinic acid hydroxylase, was induced during growth of Eubacterium barkeri on xanthine. The protein designated as xanthine dehydrogenase was enriched 39-fold to apparent homogeneity using a three-step purification scheme. It exhibited an NADP-dependent specific activity of 164 mu mol xanthine oxidized per min and per mg of protein. In addition it showed an NADPH-dependent oxidase and diaphorase activity. A molecular mass of 530 kDa was determined for the native enzyme and SDS/PAGE revealed three types of subunits with molecular masses of 17.5, 30 and 81 kDa indicating a dodecameric native structure. Molybdopterin was identified as the molybdenum-complexing cofactor using activity reconstitution experiments and fluorescence measurements after KI/I-2 oxidation. The molecular mass of the cofactor indicated that it is of the dinucleotide type. The enzyme contained iron, acid-labile sulfur, molybdenum, tungsten, selenium and FAD at molar ratios of 17.5, 18.4, 2.3, 1.1, 0.95 and 2.8 per mol of native enzyme. Xanthine dehydrogenase was inactivated upon incubation with arsenite, cyanide and different purine analogs. Reconstitution experiments of xanthine dehydrogenase activity by addition of selenide and selenite performed with cyanide-inactivated enzyme and with chloramphenicol-treated cells, respectively, indicated that selenium is not attached to the protein in a covalently bound form such as selenocysteine.
引用
收藏
页码:862 / 871
页数:10
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