Live morphological analysis of taxol-induced cytoplasmic vacuoliazation in human lung adenocarcinoma cells

被引：17

作者：

Wang, Xiao-Ping ^{[1
,2
,3
]}

Chen, Tong-Sheng ^{[1
,2
]}

Sun, Lei ^{[1
,2
]}

Cai, Ji-Ye ^{[3
]}

Wu, Ming-Qian ^{[3
]}

Mok, Martin ^{[4
]}

机构：

[1] S China Normal Univ, MOE Key Lab Laser Life Sci, Guangzhou 510631, Guangdong, Peoples R China

[2] S China Normal Univ, Inst Laser Life Sci, Guangzhou 510631, Guangdong, Peoples R China

[3] Jinan Univ, Affiliated Hosp 1, Dept Anesthesiol, Guangzhou 510632, Guangdong, Peoples R China

[4] Univ So Calif, Dept Anesthesiol, Los Angeles, CA 90033 USA

来源：

MICRON | 2008年 / 39卷 / 08期

基金：

中国国家自然科学基金;

关键词：

Taxol; Atomic force microscope (AFM); Cytoplasmic vacuolization; Confocal fluorescence microscope; Caspase-3; Endoplasmic reticulum (ER);

D O I：

10.1016/j.micron.2008.04.007

中图分类号：

TH742 [显微镜];

学科分类号：

摘要：

Taxol (paclitaxel), one of the most active cancer chemotherapeutic agents, can cause programmed cell death (PCD) and cytoplasmic vacuolization. The objective of this study was to analyze the morphological characteristics induced by taxol. Human lung adenocarcinoma (ASTC-a-1) cells were exposed to various concentration of taxol. CCK-8 was used to assay the cell viability. Atomic force microscopy (AFM), plasmid transfection and confocal fluorescence microscopy were performed to image the cells morphological change induced by taxol. Fluorescence resonance energy transfer (FRET) was used to monitor the caspase-3 activation in living cells during taxol-induced cell death. Cells treated with taxol exhibited significant swelling and cytoplasmic vacuolization which may be due to endoplasmic reticulum (ER) vacuolization. Caspase-3 was not activated during taxol-induced cytoplasmic vacuolization and cell death. These findings suggest that taxol induces caspase-3-independent cytoplasmic vacuolization, cell swelling and cell death through ER vacuolization. (C) 2008 Elsevier Ltd. All rights reserved.

引用

页码：1216 / 1221

页数：6

共 30 条

[1] Cell death independent of caspases:: A review [J].

Bröker, LE ;

Kruyt, FAE ;

Giaccone, G .

CLINICAL CANCER RESEARCH, 2005, 11 (09) :3155-3162

[2] Spatio-temporal dynamic analysis of bid activation and apoptosis induced by alkaline condition in human lung adenocarcinoma cell [J].

Chen, Tongsheng ;

Wang, Jinjun ;

Xing, Da ;

Chen, Wei R. .

CELLULAR PHYSIOLOGY AND BIOCHEMISTRY, 2007, 20 (05) :569-578

[3] Bcl-2 and Bcl-xL are important for the induction of paclitaxel resistance in human hepatocellular carcinoma cells [J].

Chun, EY ;

Lee, KY .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2004, 315 (03) :771-779

[4] The taxanes: an update [J].

Crown, J ;

O'Leary, M .

LANCET, 2000, 355 (9210) :1176-1178

[5]

Dziadyk JM, 2004, ANTICANCER RES, V24, P27

[6] Roughness of the plasma membrane as an independent morphological parameter to study RBCs: A quantitative atomic force microscopy investigation [J].

Girasole, M. ;

Pompeo, G. ;

Cricenti, A. ;

Congiu-Castellano, A. ;

Andreola, F. ;

Serafino, A. ;

Frazer, B. H. ;

Boumis, G. ;

Amiconi, G. .

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 2007, 1768 (05) :1268-1276

[7]

Huisman C, 2002, CLIN CANCER RES, V8, P596

[8] Induction of apoptosis by ASK1, a mammalian MAPKKK that activates SAPK/JNK and p38 signaling pathways [J].

Ichijo, H ;

Nishida, E ;

Irie, K ;

tenDijke, P ;

Saitoh, M ;

Moriguchi, T ;

Takagi, M ;

Matsumoto, K ;

Miyazono, K ;

Gotoh, Y .

SCIENCE, 1997, 275 (5296) :90-94

[9] Adhesion as an interplay between particle size and surface roughness [J].

Katainen, J. ;

Paajanen, M. ;

Ahtola, E. ;

Pore, V. ;

Lahtinen, J. .

JOURNAL OF COLLOID AND INTERFACE SCIENCE, 2006, 304 (02) :524-529

[10] Bcl-XL specifically activates Bak to induce swelling and restructuring of the endoplasmic reticulum [J].

Klee, M ;

Pimentel-Muiños, FX .

JOURNAL OF CELL BIOLOGY, 2005, 168 (05) :723-734

← 1 2 3 →