The human leukotriene A(4) hydrolase gene is expressed in two alternatively spliced mRNA forms

被引：15

作者：

Jendraschak, E ^{[1
]}

Kaminski, WE ^{[1
]}

Kiefl, R ^{[1
]}

vonSchacky, C ^{[1
]}

机构：

[1] UNIV MUNICH, KLINIKUM INNENSTADT, MED KLIN, D-80336 MUNICH, GERMANY

来源：

BIOCHEMICAL JOURNAL | 1996年 / 314卷

关键词：

D O I：

10.1042/bj3140733

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We identified a novel short (s-) mRNA of the human leukotriene-A(4) (LTA(4)) hydrolase using sequential reverse transcriptase PCR mapping. The s-mRNA is generated by skipping an 83 bp exon located in the 3' coding region and suggests the expression of an LTA, hydrolase isoform with a calculated molecular mass of 59 kDa and a distinct C-terminus. Both LTA(4) hydrolase mRNAs are constitutively expressed in blood cells, endothelial cells, smooth muscle cells, fibroblasts and tumour cells. The ratios of their mRNA expression levels are cell specific, with relatively high s-form mRNA expression in reticulocytes. Our data strongly suggest that the novel mRNA codes for the structurally related but distinct LTA(4) hydrolase isoenzyme that has been postulated.

引用

页码：733 / 737

页数：5

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