Simultaneous determination of lipid hydroperoxides by HPLC-post column systems

被引：8

作者：

Akasaka, K ^{[1
]}

Ohta, H ^{[1
]}

Hanada, Y ^{[1
]}

Ohrui, H ^{[1
]}

机构：

[1] Tohoku Univ, Grad Sch Agr Sci, Dept Appl Life Sci, Aoba Ku, Sendai, Miyagi 9818555, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1999年 / 63卷 / 08期

关键词：

lipid hydroperoxide; diphenyl-L-pyrenylphosphine; semi-micro high-performance liquid chromatography; post-column high-performance liquid chromatography;

D O I：

10.1271/bbb.63.1506

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Two HPLC-post column systems, a conventional-sine system and a semi-micro system, for the simultaneous determination of lipid hydroperoxides were developed. The hydroperoxides of free fatty acids, phosphatidylcholines, triacylglycerols and cholesterol esters were individually determined at the pmol level with good reproducibility by using gradient elution and post-column detection with diphenyl-1-pyrenylphosphine.

引用

页码：1506 / 1508

页数：3

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