Tissue molecular ion imaging by gold cluster ion bombardment

被引：228

作者：

Touboul, D

Halgand, F

Brunelle, A

Kersting, R

Tallarek, E

Hagenhoff, B

Laprévote, O

机构：

[1] CNRS, Inst Chim Subst Nat, F-91198 Gif Sur Yvette, France

[2] Tascon GmbH, D-48149 Munster, Germany

来源：

ANALYTICAL CHEMISTRY | 2004年 / 76卷 / 06期

关键词：

D O I：

10.1021/ac035243z

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The use of gold cluster focused ion beams produced by a liquid metal ion gun in a TOF-SIMS mass spectrometer is shown to dramatically enhance secondary ion emission of phospholipids and peptides. The method has been successfully tested with cells grown onto plastic slips and with mouse brain slices, without any treatment of the samples. Very reliable time-of-flight mass spectra are acquired with a low primary ion dose of a few 107 ions, and high lateral resolution molecular ion images are obtained for heavy ions of great biological interest. This approach offers new opportunities in pharmacological and biological research fields by localizing compounds of interest such as drugs or metabolites in tissues.

引用

收藏

页码：1550 / 1559

页数：10

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