Protein-DNA interaction: impedance study of MutS binding to a DNA mismatch

被引：44

作者：

Li, CZ

Long, YT

Lee, JS

Kraatz, HB

机构：

[1] Univ Saskatchewan, Dept Biochem, Saskatoon, SK S7N 5E5, Canada

[2] Univ Saskatchewan, Dept Chem, Saskatoon, SK S7N 5C9, Canada

来源：

CHEMICAL COMMUNICATIONS | 2004年 / 05期

关键词：

D O I：

10.1039/b314642a

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

MutS binding to a double-stranded DNA containing a single nucleotide mismatch can be conveniently monitored by impedance spectroscopy and represents the first step in developing an electrochemical binding essay for single nucleotide mismatch detection.

引用

收藏

页码：574 / 575

页数：2

相关论文

共 22 条

[1] Liposomes labeled with biotin and horseradish peroxidase: A probe for the enhanced amplification of antigen-antibody or oligonucleotide-DNA sensing processes by the precipitation of an insoluble product on electrodes [J].

Alfonta, L ;

Singh, AK ;

Willner, I .

ANALYTICAL CHEMISTRY, 2001, 73 (01) :91-102

[2] Chronopotentiometry and Faradaic impedance spectroscopy as signal transduction methods for the biocatalytic precipitation of an insoluble product on electrode supports: routes for enzyme sensors, immunosensors and DNA sensors [J].

Alfonta, L ;

Bardea, A ;

Khersonsky, O ;

Katz, E ;

Willner, I .

BIOSENSORS & BIOELECTRONICS, 2001, 16 (9-12) :675-687

[3] Mutation detection by electrocatalysis at DNA-modified electrodes [J].

Boon, EM ;

Ceres, DM ;

Drummond, TG ;

Hill, MG ;

Barton, JK .

NATURE BIOTECHNOLOGY, 2000, 18 (10) :1096-1100

[4] An electrical probe of protein-DNA interactions on DNA-modified surfaces [J].

Boon, EM ;

Salas, JE ;

Barton, JK .

NATURE BIOTECHNOLOGY, 2002, 20 (03) :282-286

[5] Effect of hexacyanoferrate(II/III) on self-assembled monolayers of thioctic acid and 11-mercaptoundecanoic acid on gold [J].

Dijksma, M ;

Boukamp, BA ;

Kamp, B ;

van Bennekom, WP .

LANGMUIR, 2002, 18 (08) :3105-3112

[6] MULTIPLEXED BIOCHEMICAL ASSAYS WITH BIOLOGICAL CHIPS [J].

FODOR, SPA ;

RAVA, RP ;

HUANG, XHC ;

PEASE, AC ;

HOLMES, CP ;

ADAMS, CL .

NATURE, 1993, 364 (6437) :555-556

[7] DNA mismatch repair and genetic instability [J].

Harfe, BD ;

Jinks-Robertson, S .

ANNUAL REVIEW OF GENETICS, 2000, 34 :359-399

[8] Electrochemical study of electron transport through thin DNA films [J].

Hartwich, G ;

Caruana, DJ ;

de Lumley-Woodyear, T ;

Wu, YB ;

Campbell, CN ;

Heller, A .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1999, 121 (46) :10803-10812

[9] Preliminary evaluation of electrochemical PNA array for detection of single base mismatch mutations [J].

Hashimoto, K ;

Ishimori, Y .

LAB ON A CHIP, 2001, 1 (01) :61-63

[10] Gene sensor using ferrocenyl oligonucleotide [J].

Ihara, T ;

Nakayama, M ;

Murata, M ;

Nakano, K ;

Maeda, M .

CHEMICAL COMMUNICATIONS, 1997, (17) :1609-1610