Staphylococcus aureus alpha toxin mediates polymorphonuclear leukocyte-induced vasocontraction and endothelial dysfunction

被引:14
作者
Buerke, M
Sibelius, U
Grandel, U
Buerke, U
Grimminger, F
Seeger, W
Meyer, J
Darius, H
机构
[1] Johannes Gutenberg Univ Mainz, Dept Med, D-55101 Mainz, Germany
[2] Univ Giessen, Dept Internal Med, D-35393 Giessen, Germany
来源
SHOCK | 2002年 / 17卷 / 01期
关键词
Staphylococcus aureus alpha-toxin; neutrophil adherence; endothelial dysfunction; selectin; nitric oxide; fucoidin;
D O I
10.1097/00024382-200201000-00006
中图分类号
R4 [临床医学];
学科分类号
1002 ; 100602 ;
摘要
The effect of Staphylococcus aureus alpha toxin (alpha -toxin) on selectin-mediated neutrophil adhesion was investigated in polymorphonuclear leukocyte- (PMN) induced vasocontraction and endothelial dysfunction, Adherence of human PMNs to rat aortic endothelium increased significantly following stimulation of the endothelium with alpha -toxin (0.1, 0.5, and 1 mug/mL). This effect could be significantly attenuated by monoclonal antibodies directed against P-selectin or fucoidin, a carbohydrate known to block selectins. Unstimulated human PMNs (10(6) cells/mL) were added to organ chambers containing rat aortic rings stimulated with alpha -toxin (0.5 mug/mL). PMNs elicited a significant vasocontraction in alpha -toxin-stimulated, but not in control aortic, rings (142 +/- 12 mg versus 12 +/- 4 mg, P < 0.05). This PMN-induced vasocontraction was virtually blunted by pretreatment with MAb directed against P-selectin or fucoidin (P < 0.05). Endothelial function as assessed by endothelium-dependent vasorelaxation to acetylcholine was substantially inhibited after induction of PMN-induced vasocontraction in alpha -toxin-stimulated aortic rings. This endothelial dysfunction was reduced by P-selectin MAb or fucoidin. In contrast, endothelium-independent relaxation to sodium nitrite was not altered by PMN incubation, indicating that vascular smooth muscle function was unaffected. Thus, PMN-endothelial interaction following S. aureus alpha -toxin activation of the vascular endothelium is at least, in part, mediated by selectins. As a consequence, PMN-induced vasocontraction and endothelial dysfunction occur. Such mechanisms may be involved in microcirculation abnormalities encountered in sepsis or septic shock due to S. aureus infection.
引用
收藏
页码:30 / 35
页数:6
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