Genome-Wide Control of the Distribution of Meiotic Recombination

被引:57
作者
Grey, Corinne [1 ]
Baudat, Frederic [1 ]
de Massy, Bernard [1 ]
机构
[1] CNRS, UPR1142, Inst Human Genet, Montpellier, France
来源
PLOS BIOLOGY | 2009年 / 7卷 / 02期
关键词
MAJOR HISTOCOMPATIBILITY COMPLEX; DOUBLE-STRAND BREAKS; HOTSPOT CONVERSION PARADOX; HOT-SPOTS; SACCHAROMYCES-CEREVISIAE; CHROMATIN-STRUCTURE; FISSION YEAST; MOLECULAR CHARACTERIZATION; MOUSE SPERMATOCYTES; CROSSOVER HOTSPOTS;
D O I
10.1371/journal.pbio.1000035
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Meiotic recombination events are not randomly distributed in the genome but occur in specific regions called recombination hotspots. Hotspots are predicted to be preferred sites for the initiation of meiotic recombination and their positions and activities are regulated by yet-unknown controls. The activity of the Psmb9 hotspot on mouse Chromosome 17 (Chr 17) varies according to genetic background. It is active in strains carrying a recombinant Chr 17 where the proximal third is derived from Mus musculus molossinus. We have identified the genetic locus required for Psmb9 activity, named Dsbc1 for Double-strand break control 1, and mapped this locus within a 6.7-Mb region on Chr 17. Based on cytological analysis of meiotic DNA double-strand breaks (DSB) and crossovers (COs), we show that Dsbc1 influences DSB and CO, not only at Psmb9, but in several other regions of Chr 17. We further show that CO distribution is also influenced by Dsbc1 on Chrs 15 and 18. Finally, we provide direct molecular evidence for the regulation in trans mediated by Dsbc1, by showing that it controls the CO activity at the Hlx1 hotspot on Chr 1. We thus propose that Dsbc1 encodes for a trans-acting factor involved in the specification of initiation sites of meiotic recombination genome wide in mice.
引用
收藏
页码:327 / 339
页数:13
相关论文
共 64 条
  • [21] Comparative recombination rates in the rat, mouse, and human genomes
    Jensen-Seaman, MI
    Furey, TS
    Payseur, BA
    Lu, YT
    Roskin, KM
    Chen, CF
    Thomas, MA
    Haussler, D
    Jacob, HJ
    [J]. GENOME RESEARCH, 2004, 14 (04) : 528 - 538
  • [22] Infrequent co-conversion of markers flanking a meiotic recombination initiation site in Saccharomyces cerevisiae
    Jessop, L
    Allers, T
    Lichten, M
    [J]. GENETICS, 2005, 169 (03) : 1353 - 1367
  • [23] Mechanism and control of meiotic recombination initiation
    Keeney, S
    [J]. CURRENT TOPICS IN DEVELOPMENTAL BIOLOGY, VOL 52, 2001, 52 : 1 - 53
  • [24] Ea recombinational hot spot in the mouse major histocompatibility complex maps to the fourth intron of the Ea gene
    Khambata, S
    Mody, J
    Modzelewski, A
    Heine, D
    Passmore, HC
    [J]. GENOME RESEARCH, 1996, 6 (03): : 195 - 201
  • [25] A high-resolution recombination map of the human genome
    Kong, A
    Gudbjartsson, DF
    Sainz, J
    Jonsdottir, GM
    Gudjonsson, SA
    Richardsson, B
    Sigurdardottir, S
    Barnard, J
    Hallbeck, B
    Masson, G
    Shlien, A
    Palsson, ST
    Frigge, ML
    Thorgeirsson, TE
    Gulcher, JR
    Stefansson, K
    [J]. NATURE GENETICS, 2002, 31 (03) : 241 - 247
  • [26] RECOMBINATION HOT-SPOTS WITHIN THE I-REGION OF THE MOUSE H-2-COMPLEX MAP TO THE E-BETA AND E-ALPHA GENES
    LAFUSE, WP
    DAVID, CS
    [J]. IMMUNOGENETICS, 1986, 24 (06) : 352 - 360
  • [27] Extreme heterogeneity in the molecular events leading to the establishment of chiasmata during meiosis I in human oocytes
    Lenzi, ML
    Smith, J
    Snowden, T
    Kim, M
    Fishel, R
    Poulos, BK
    Cohen, PE
    [J]. AMERICAN JOURNAL OF HUMAN GENETICS, 2005, 76 (01) : 112 - 127
  • [28] Lichten Michael, 2008, V3, P165, DOI 10.1007/7050_2008_040
  • [29] Variation inhuman meiotic recombination
    Lynn, A
    Ashley, T
    Hassold, T
    [J]. ANNUAL REVIEW OF GENOMICS AND HUMAN GENETICS, 2004, 5 : 317 - 349
  • [30] ZMM proteins during meiosis:: Crossover artists at work
    Lynn, Audrey
    Soucek, Rachel
    Boerner, G. Valentin
    [J]. CHROMOSOME RESEARCH, 2007, 15 (05) : 591 - 605