Inhibition of the vacuolating and anion channel activities of the VacA toxin of Helicobacter pylori

被引:59
作者
Tombola, F
Oregna, F
Brutsche, S
Szabò, I
Del Giudice, G
Rappuoli, R
Montecucco, C
Papini, E
Zoratti, M
机构
[1] Univ Padua, CNR, Ctr Biomembrane, Dipartimento Sci Biomed, I-35121 Padua, Italy
[2] CHIRON Vaccines, Ctr Ric IRIS, I-53100 Siena, Italy
[3] Univ Bari, Dipartimento Sci Biomed & Oncol Umana, I-70100 Bari, Italy
关键词
gastritis; ulcer; VacA; ion channel inhibition; Helicobacter pylori;
D O I
10.1016/S0014-5793(99)01348-4
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
VacA, the vacuolating cytotoxin secreted by Helicobacter pylori, is believed to be a major causative factor in the development of gastroduodenal ulcers, This toxin causes vacuolation of cultured cells and it has recently been found to form anion-selective channels upon insertion into planar bilayers as well as in the plasmamembrane of HeLa cells. Here, we identify a series of inhibitors of VacA channels and we compare their effectiveness as channel blockers and as inhibitors of VacA-induced vacuolation, confirming that the two phenomena are linked, This characterization opens the way to studies in other experimental systems and to the search for a specific inhibitor of VacA action. (C) 1999 Federation of European Biochemical Societies.
引用
收藏
页码:221 / 225
页数:5
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