Intra- and intercellular trafficking of the foamy virus auxiliary bet protein

被引:30
作者
Lecellier, CH
Vermeulen, W
Bachelerie, F
Giron, ML
Saïb, A
机构
[1] Univ Paris 07, Hop St Louis, CNRS, UPR9051, F-75475 Paris 10, France
[2] Inst Pasteur, Unite Immunol Virale, F-75724 Paris, France
[3] Erasmus Univ, Dept Genet, Rotterdam, Netherlands
关键词
D O I
10.1128/JVI.76.7.3388-3394.2002
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The Bet protein of foamy viruses (FVs) is an auxiliary protein encoded by the 3' end of the viral genome. Although its function during the viral replication cycle is still unknown, Bet seems to play a key role in the establishment and/or maintenance of viral persistence, representing the predominant viral protein detected during chronic infection. To clarify the function of this viral protein, the subcellular distribution of Bet from the prototypic human foamy virus (HFV) was examined. We report here that this protein is distributed in both the cytoplasm and the nucleus of HFV-infected or Bet-transfected cells. The nuclear targeting results from the presence of a bipartite nuclear localization signal at the C-terminal region, sufficient to direct heterologous reporter proteins to the nucleus. Since HFV Bet spreads between cells, we show here that the secreted protein targets the nuclei of recipient cells. HFV Bet follows an unconventional route to exit the cell since its secretion is not affected by brefeldin A, a drug which disrupts the trafficking between the endoplasmic reticulum and the Golgi complex. Finally, these inter- and intracellular movements were also observed for the equine foamy virus Bet protein, strong suggesting that these remarkable features are conserved among FVs.
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页码:3388 / 3394
页数:7
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