Lipid-PEG-Folate Encapsulated Nanoparticles with Aggregation Induced Emission Characteristics: Cellular Uptake Mechanism and Two-Photon Fluorescence Imaging

被引：191

作者：

Geng, Junlong ^{[1
]}

Li, Kai ^{[2
]}

Ding, Dan ^{[1
]}

Zhang, Xinhai ^{[2
]}

Qin, Wei ^{[3
,4
]}

Liu, Jianzhao ^{[3
,4
]}

Tang, Ben Zhong ^{[2
,3
,4
]}

Liu, Bin ^{[1
,2
]}

机构：

[1] Natl Univ Singapore, Dept Chem & Biomol Engn, Singapore 117576, Singapore

[2] Inst Mat Res Engn, Singapore 117602, Singapore

[3] Hong Kong Univ Sci & Technol, Inst Mol Funct Mat, Kowloon, Hong Kong, Peoples R China

[4] Hong Kong Univ Sci & Technol, State Key Lab Mol Neurosci, Div Biomed Engn, Dept Chem,Inst Adv Study, Kowloon, Hong Kong, Peoples R China

来源：

SMALL | 2012年 / 8卷 / 23期

基金：

新加坡国家研究基金会;

关键词：

two-photon absorption; aggregation-induced emission; fluorescence imaging; cellular uptake mechanism; folic acid; IN-VIVO; CONJUGATED POLYELECTROLYTE; ENHANCED FLUORESCENCE; POLYMER NANOPARTICLES; QUANTUM DOTS; ABSORPTION; CHROMOPHORES; DELIVERY; MOLECULES; CANCER;

D O I：

10.1002/smll.201200814

中图分类号：

O6 [化学];

学科分类号：

070301 [无机化学];

摘要：

Folate functionalized nanoparticles (NPs) that contain fluorogens with aggregation-induced emission (AIE) characteristics are fabricated to show bright far-red/near-infrared fluorescence, a large two-photon absorption cross section and low cytotoxicity, which are internalized into MCF-7 cancer cells mainly through caveolae-mediated endocytosis. One-photon excited in vivo fluorescence imaging illustrates that these AIE NPs can accumulate in a tumor and two-photon excited ex vivo tumor tissue imaging reveals that they can be easily detected in the tumor mass at a depth of 400 mu m. These studies indicate that AIE NPs are promising alternatives to conventional TPA probes for biological imaging.

引用

页码：3655 / 3663

页数：9

共 51 条

[1]

Design of organic molecules with large two-photon absorption cross sections [J].

Albota, M ;

Beljonne, D ;

Brédas, JL ;

Ehrlich, JE ;

Fu, JY ;

Heikal, AA ;

Hess, SE ;

Kogej, T ;

Levin, MD ;

Marder, SR ;

McCord-Maughon, D ;

Perry, JW ;

Röckel, H ;

Rumi, M ;

Subramaniam, C ;

Webb, WW ;

Wu, XL ;

Xu, C .

SCIENCE, 1998, 281 (5383) :1653-1656

[2]

POTOCYTOSIS - SEQUESTRATION AND TRANSPORT OF SMALL MOLECULES BY CAVEOLAE [J].

ANDERSON, RGW ;

KAMEN, BA ;

ROTHBERG, KG ;

LACEY, SW .

SCIENCE, 1992, 255 (5043) :410-411

[3]

A role for phosphoinositide 3-kinase in the completion of macropinocytosis and phagocytosis by macrophages [J].

Araki, N ;

Johnson, MT ;

Swanson, JA .

JOURNAL OF CELL BIOLOGY, 1996, 135 (05) :1249-1260

[4]

Determining the size and shape dependence of gold nanoparticle uptake into mammalian cells [J].

Chithrani, BD ;

Ghazani, AA ;

Chan, WCW .

NANO LETTERS, 2006, 6 (04) :662-668

[5]

Conjugated polyelectrolyte-cisplatin complex nanoparticles for simultaneous in vivo imaging and drug tracking [J].

Ding, Dan ;

Li, Kai ;

Zhu, Zhenshu ;

Pu, Kan-Yi ;

Hu, Yong ;

Jiang, Xiqun ;

Liu, Bin .

NANOSCALE, 2011, 3 (05) :1997-2002

[6]

Mechanisms of Endocytosis [J].

Doherty, Gary J. ;

McMahon, Harvey T. .

ANNUAL REVIEW OF BIOCHEMISTRY, 2009, 78 :857-902

[7]

PHOTOPHYSICS AND CIS-TRANS ISOMERIZATION OF DCM [J].

DRAKE, JM ;

LESIECKI, ML ;

CAMAIONI, DM .

CHEMICAL PHYSICS LETTERS, 1985, 113 (06) :530-534

[8]

Conjugated Polymer and Gold Nanoparticle Co-loaded PLGA Nanocomposites with Eccentric Internal Nanostructure for Dual-modal Targeted Cellular Imaging [J].

Geng, Junlong ;

Li, Kai ;

Pu, Kan-Yi ;

Ding, Dan ;

Liu, Bin .

SMALL, 2012, 8 (15) :2421-2429

[9]

GOTTSCHALK S, 1994, GENE THER, V1, P185

[10]

Multiphoton absorbing materials: Molecular designs, characterizations, and applications [J].

He, Guang S. ;

Tan, Loon-Seng ;

Zheng, Qingdong ;

Prasad, Paras N. .

CHEMICAL REVIEWS, 2008, 108 (04) :1245-1330

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