Understanding mutations and protein stability through tripeptides

被引:7
作者
Anishetty, S
Anishetty, R [1 ]
Pennathur, G
机构
[1] Anna Univ, Ctr Biotechnol, Madras 600025, Tamil Nadu, India
[2] Inst Math Sci, Madras 600113, Tamil Nadu, India
[3] Anna Univ, AU KBC Res Ctr, Madras 600044, Tamil Nadu, India
关键词
SNPs; missense mutation; tripeptide; protein structure; disease; structural biology;
D O I
10.1016/j.febslet.2006.02.079
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A novel methodology to predict the local conformational changes in a protein as a consequence of missense mutations is proposed. A pentapeptide at the locus of mutation plays the dominant role and it is analyzed in terms of tripeptides. A measure for spatial and temporal fluctuations in a pentapeptide is devised and validated. The method does not involve any prior knowledge of structural templates from sequence homology studies. Structural deformations can be predicted with about 70-80%. reliability in any protein. Disease causing mutations and benign mutations have been addressed. In particular, p53, retinoblastoma protein and lipoprotein lipase are studied in detail. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
引用
收藏
页码:2071 / 2080
页数:10
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