Peroxynitrite-modified DNA: a better antigen for systemic lupus erythematosus anti-DNA autoantibodies

被引：22

作者：

Habib, S ^{[1
]}

Ali, M ^{[1
]}

Ali, R ^{[1
]}

机构：

[1] Aligarh Muslim Univ, Jawaharlal Nehru Med Coll, Dept Biochem, Aligarh 202002, Uttar Pradesh, India

来源：

BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY | 2006年 / 43卷

关键词：

anti-DNA autoantibody antigen; DNA damage; peroxynitrite; (ONOO-); peroxynitrite-modified DNA; systemic lupus erythematosus;

D O I：

10.1042/BA20050156

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

Native human DNA was modified by ONOO- (peroxynitrite), generated by the synergistic action of sodium nitroprusside [Na2Fe(CN)(5)NO], an NO donor, and pyrogallol, a superoxide donor. The modifications were analysed by UV absorption characteristics, fluorescence emission transitions, nuclease S1 digestibility and melting-temperature studies. Modified DNA was found to be highly immunogenic, inducing high-titre immunogen-specific antibodies in experimental animals. A maximum of 85% inhibition of the antibody binding was observed in competition ELISA with immunogen as inhibitor. SLE (systemic lupus erythematosus) anti-DNA autoantibodies recognized modified DNA as a better antigen than the native analogue. Modification of native DNA by ONOO-, forming neo-epitopes on the molecule, may be one of the factors for the induction of the autoimmune response seen in SLE.

引用

页码：65 / 70

页数：6

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