Purification and properties of poly(3-hydroxybutyrate) depolymerase from the fungus Paecilomyces lilacinus D218

被引：44

作者：

Oda, Y ^{[1
]}

Osaka, H ^{[1
]}

Urakami, T ^{[1
]}

Tonomura, K ^{[1
]}

机构：

[1] MITSUBISHI GAS CHEM CO,CHIYODA KU,TOKYO 100,JAPAN

来源：

CURRENT MICROBIOLOGY | 1997年 / 34卷 / 04期

关键词：

D O I：

10.1007/s002849900174

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Poly(3-hydroxybutyrate) depolymerase was purified to homogeneity from the culture filtrate of Paecilomyces lilacinus D218 by column chromatography on CM-Toyopearl 650M and hydroxylapatite. The molecular weight of the enzyme was estimated to be 48,000 by SDS-PAGE. Maximal activity was observed near pH 7.0 ans 45 degrees C. The K-m and V-max walues for PHB were 0.13 (mg/ml) and 3750 (U/mg protein), respectively. The enzyme hydrolyzed PHB and p-nitrophenyl fatty acids but not polycaprolactone and triglycerides.

引用

页码：230 / 232

页数：3

共 13 条

[1]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[2] EXTRACELLULAR POLY(3-HYDROXYBUTYRATE) DEPOLYMERASE FROM PENICILLIUM-FUNICULOSUM - GENERAL-CHARACTERISTICS AND ACTIVE-SITE STUDIES [J].