Immunoaffinity solid-phase extraction for pharmaceutical and biomedical trace-analysis-coupling with HPLC and CE-perspectives

Immunoaffinity solid-phase extraction (SPE) technique is based upon a molecular recognition mechanism. The high affinity and the high selectivity of the antigen-antibody interactions allow the specific extraction and the concentration of the analytes of interest in one step. In pharmaceutical and biological fields, where most often matrices are complex and analytes at trace-levels, this approach constitutes a unique tool for fast and solvent-free sample preparation. This review presents a general description of this extraction technique and gives numerous examples of its applications in pharmaceutical and biomedical fields. It emphasizes the on-line coupling with chromatographic and electrophoretic separation techniques and introduces new developments. The future directions, especially with regards to the current development of analytical microsystems, are discussed. (C) 2003 Elsevier B.V. All rights reserved.