Association of the cell cycle transcription factor Mbp1 with the Skn7 response regulator in budding yeast

被引:50
作者
Bouquin, N [1 ]
Johnson, AL [1 ]
Morgan, BA [1 ]
Johnston, LH [1 ]
机构
[1] Natl Inst Med Res, Ridgeway, Div Yeast Genet, London NW7 1AA, England
基金
英国惠康基金;
关键词
D O I
10.1091/mbc.10.10.3389
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
We previously isolated the SKN7 gene in a screen designed to isolate new components of the G1-S cell cycle transcription machinery in budding yeast. We have now found that Skn7 associates with Mbp1, the DNA-binding component of the G1-S transcription factor DSC1/MBF. SKN7 and MBP1 show several genetic interactions. Skn7 overexpression is lethal and is suppressed by a mutation in MBP1. Similarly, high overexpression of Mbp1 is lethal and can be suppressed by skn7 mutations. SKN7 is also required for MBP1 function in a mutant compromised for G1-specific transcription. Gel-retardation assays indicate that Skn7 is not an integral part of MBF. However, a physical interaction between Skn7 and Mbp1 was detected using two-hybrid assays and GST pulldowns. Thus, Skn7 and Mbp1 seem to form a transcription factor independent of MBF. Genetic data suggest that this new transcription factor could be involved in the bud-emergence process.
引用
收藏
页码:3389 / 3400
页数:12
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