A novel function of CEP135 as a platform protein of C-NAP1 for its centriolar localization

被引：52

作者：

Kim, Kyeongmi

Lee, Seongju

Chang, Jaerak

Rhee, Kunsoo ^{[1
]}

机构：

[1] Seoul Natl Univ, Dept Biol Sci, Seoul 151747, South Korea

来源：

EXPERIMENTAL CELL RESEARCH | 2008年 / 314卷 / 20期

关键词：

Centrosome; CEP135; C-NAP1; Centrosome split;

D O I：

10.1016/j.yexcr.2008.09.016

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

A proteomic study predicted that about one hundred kinds of proteins constitute a basic structure of the centrosome. Most of the core centrosomal proteins contain extensive coiled-coil domains, suggesting that the protein-protein interaction is a Critical force for the core centrosome configuration. In the present study, we investigated a novel interaction between CEP135 and C-NAP1, two core centriolar proteins. Depletion of CEP135 caused a premature centrosome splitting. Reduction of the centrosomal C-NAP1 level was accompanied in a specific manner. Ectopic expression of the CEP135 Mutant proteins also caused centrosome splitting in association with the reduction of the centrosomal C-NAP1 levels. Based on these results, we propose that CEP135 acts as a platform protein for C-NAP1 at the centriole. (c) 2008 Elsevier Inc. All rights reserved.

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收藏

页码：3692 / 3700

页数：9

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