Hematopoietic-specific microRNA expression in human cells

被引：195

作者：

Ramkissoon, SH

Mainwaring, LA

Ogasawara, Y

Keyvanfar, K

McCoy, JP

Sloand, EM

Kajigaya, S

Young, NS

机构：

[1] NHLBI, Hematol Branch, NIH, Bethesda, MD 20892 USA

[2] NIH, Howard Hughes Med Inst, Res Scholars Program, Bethesda, MD 20892 USA

[3] Univ Med & Dent New Jersey, Grad Sch Biomed Sci, Newark, NJ 07109 USA

[4] NHLBI, Flow Cytometry Core Facil, NIH, Bethesda, MD 20892 USA

来源：

LEUKEMIA RESEARCH | 2006年 / 30卷 / 05期

关键词：

microRNA; hematopoietic cells; leukemic cells; FACS; northem analysis;

D O I：

10.1016/j.leukres.2005.09.001

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

We examined expression profiles of hematopoietic tissue-specific microRNAs (miRNAs; rniR-142, miR-155, miR-181 and miR-223) in 17 commercially available malignant hematopoietic cell lines and compared to those in highly purified normal human B. T, monocytic and granulocytic lineages. Although malignant cell lines examined showed miRNA expression patterns similar to normal human hematopoietic lineages, the levels of miRNA expression among cell lines and normal cell lineages were considerably different, indicating the significance of miRNAs in human hematopoietic diseases. Further our results showed differences in miRNA expression between mouse and human hematopoietic cells, suggesting important regulatory roles of miRNAs in human hematopoiesis and oncogenesis. (C) 2005 Elsevier Ltd. All rights reserved.

引用

页码：643 / 647

页数：5

共 8 条

[1] MicroRNAs: Genomics, biogenesis, mechanism, and function (Reprinted from Cell, vol 116, pg 281-297, 2004) [J].