Amperometric glucose biosensor based on layer-by-layer covalent attachment of AMWNTs and IO4--oxidized GOx

被引:43
作者
Sun, Yingying [1 ]
Wang, Haiying [1 ]
Sun, Changqing [1 ]
机构
[1] Jilin Univ, Coll Chem, Changchun 130012, Peoples R China
关键词
amino terminated carbon nanotubes; layer-by-layer covalent attachment; low-potential detection of the glucose; glucose biosensors;
D O I
10.1016/j.bios.2008.03.004
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
Multi-wall carbon nanotubes (MWNTs) functionalized with amino groups were prepared via silane treatment using 3-aminopropyltrimethoxysilane (APS) as a silane-coupling agent. The resultant amino terminated MWNTs (AMWNTs) were applied to construct glucose biosensors with IO4--oxidized glucose oxidase (IO4--oxidized GOx) through the layer-by-layer (LBL) covalent self-assembly method without any cross-linker. Scanning electron microscopy (SEM) indicated that the assembled AMWNTs were almost in a form of small bundles or single nanotubes, and the surface density increased uniformly with the number of GOx/AMWNTs bilayers, From the analysis of voltammetric signals, a linear increment of the coverage of GOx per bilayer was estimated. The resulting biosensor showed excellent catalytic activity towards the electroreduction of dissolved oxygen at low overvoltage, based on which glucose concentration was monitored conveniently. The enzyme electrode exhibited good electrocatalytic response towards the glucose and that response increased with the number of GOx/AMWNTs bilayers, suggesting that the analytical performance such as sensitivity and detection limit of the glucose biosensors could be tuned to the desired level by adjusting the number of deposited GOx/AMWNTs bilayers. The biosensor constructed with four bilayers of GOx/AMWNTs showed high sensitivity of 7.46 mu A mM(-1) cm(-2) and the detection limit of 8.0 mu M, with a fast response less than 10 s. Because of relative low applied potential, the interference from other electro-oxidizable compounds was minimized, which improved the selectivity of the biosensors. Furthermore, the obtained enzyme electrodes also showed remarkable stability due to the covalent interaction between the GOx and AMWNTs. (C) 2008 Elsevier B.V. All rights reserved.
引用
收藏
页码:22 / 28
页数:7
相关论文
共 49 条
  • [1] THE DESIGN OF ENZYME SENSORS BASED ON THE ENZYME STRUCTURE
    ALVAREZICAZA, M
    KALISZ, HM
    HECHT, HJ
    AUMANN, KD
    SCHOMBURG, D
    SCHMID, RD
    [J]. BIOSENSORS & BIOELECTRONICS, 1995, 10 (08) : 735 - 742
  • [2] Electron transfer in organized assemblies of biomolecules.: Step-by-step avidin/biotin construction and dynamic characteristics of a spatially ordered multilayer enzyme electrode
    Anicet, N
    Bourdillon, C
    Moiroux, J
    Savéant, JM
    [J]. JOURNAL OF PHYSICAL CHEMISTRY B, 1998, 102 (49): : 9844 - 9849
  • [3] BARD AJ, 1980, ELECTROCHEMICAL METH
  • [4] Carbon nanotubes - the route toward applications
    Baughman, RH
    Zakhidov, AA
    de Heer, WA
    [J]. SCIENCE, 2002, 297 (5582) : 787 - 792
  • [5] Bockris J.O., 1969, Fuel Cells: Their electrochemistry
  • [6] A FULLY ACTIVE MONOLAYER ENZYME ELECTRODE DERIVATIZED BY ANTIGEN-ANTIBODY ATTACHMENT
    BOURDILLON, C
    DEMAILLE, C
    GUERIS, J
    MOIROUX, J
    SAVEANT, JM
    [J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1993, 115 (26) : 12264 - 12269
  • [7] Faradaic impedance behaviour of oxidized and reduced poly (2,5-di-(-2-thienyl)-thiophene) films
    Brillas, E
    Cabot, PL
    Garrido, JA
    Montilla, M
    Rodriguez, RM
    Carrasco, J
    [J]. JOURNAL OF ELECTROANALYTICAL CHEMISTRY, 1997, 430 (1-2): : 133 - 140
  • [8] Britto PJ, 1999, ADV MATER, V11, P154, DOI 10.1002/(SICI)1521-4095(199902)11:2<154::AID-ADMA154>3.0.CO
  • [9] 2-B
  • [10] Direct electrochemistry and electrocatalysis of horseradish peroxidase immobilized in sol-gel-derived ceramic-carbon nanotube nanocomposite film
    Chen, Hongjun
    Dong, Shaojun
    [J]. BIOSENSORS & BIOELECTRONICS, 2007, 22 (08) : 1811 - 1815