Diversity of bacterial community in freshwater of Woopo wetland

被引：26

作者：

Baik, Keun Sik ^{[1
]}

Park, Seong Chan ^{[1
]}

Kim, Eun Mi ^{[1
,2
]}

Bae, Kyung Sook ^{[3
]}

Ann, Jae-Hyung ^{[4
]}

Ka, Jong-Ok ^{[4
]}

Chun, Jongsik ^{[5
]}

Seong, Chi Nam ^{[1
]}

机构：

[1] Sunchon Natl Univ, Dept Biol, Sunchon 540742, South Korea

[2] Gwangju Hlth Coll Univ, Dept Dent Hyg, Kwangju 506701, South Korea

[3] Korea Res Inst Biosci & Biotechnol, Biol Resource Ctr, Taejon 305333, South Korea

[4] Seoul Natl Univ, Dept Agr Biotechnol, Seoul 151742, South Korea

[5] Seoul Natl Univ, Sch Biol Sci, Seoul 151742, South Korea

来源：

JOURNAL OF MICROBIOLOGY | 2008年 / 46卷 / 06期

关键词：

diversity; Woopo wetland; bacterial isolates; 16S rRNA gene clones; phylotypes;

D O I：

10.1007/s12275-008-0135-x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Diversity of bacterial community in water layer of Woopo wetland was investigated. Cultivable bacterial strains were isolated by the standard dilution plating technique and culture-independent 16S rRNA gene clones were obtained directly from DNA extracts of a water sample. Amplified rDNA restriction analysis (ARDRA) was applied onto both of the isolates and 16S rRNA gene clones. Rarefaction curves, coverage rate and diversity indices of ARDRA patterns were calculated. Representative isolates and clones of all the single isolate/clone phylotype were partially sequenced and analyzed phylogenetically. Sixty-four and 125 phylotypes were obtained from 203 bacterial isolates and 235 culture-independent 16S rRNA gene clones, respectively. Bacterial isolates were composed of 4 phyla, of which Firmicutes (49.8%) and Actinobacteria (32.0%) were predominant. Isolates were affiliated with 58 species. Culture-independent 16S rRNA gene clones were composed of 8 phyla, of which Proteobacteria (62.2%), Actinobacteria (15.5%), and Bacteroidetes (13.7%) were predominant. Diversity of 16S rRNA gene clones originated from cultivation-independent DNA extracts was higher than that of isolated bacteria.

引用

页码：647 / 655

页数：9

共 49 条

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