A high-resolution structure of ligand-free human glutamate carboxypeptidase II

被引：41

作者：

Barinka, Cyril ^{[1
]}

Starkova, Jana

Konvalinka, Jan

Lubkowski, Jacek

机构：

[1] Ctr Canc Res, Natl Canc Inst Frederick, Frederick, MD 21702 USA

[2] Acad Sci Czech Republ, Gilead Sci Inc, CZ-6610 Prague 6, Czech Republic

[3] Acad Sci Czech Republ, IOCB Res Ctr, CZ-6610 Prague 6, Czech Republic

[4] Charles Univ Prague, Dept Biochem, Fac Nat Sci, CZ-2030 Prague, Czech Republic

来源：

ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS | 2007年 / 63卷

关键词：

D O I：

10.1107/S174430910700379X

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Human glutamate carboxypeptidase II ( GCPII; EC 3.4.17.21) is an established marker for prostate-cancer diagnosis as well as a candidate therapeutic target for the treatment of diverse pathologies that involve glutamatergic transmission. Structural data on GCPII are thus valuable for the design and optimization of GCPII-specific inhibitors and diagnostic probes. The currently available structure of ligand-free GCPII was refined to a resolution of 3.5 angstrom. This work reports the structure of the protein refined to 1.65 angstrom resolution, with crystallographic values of R = 0.207 and R-free = 0.228. The new structure extends the resolution appreciably and the new model based on this data shows significant differences when compared with the previously published model.

引用

页码：150 / 153

页数：4

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