Effects of mutating putative two-component systems on biofilm formation by Streptococcus mutans UA159

被引：57

作者：

Bhagwat, SP ^{[1
]}

Nary, J ^{[1
]}

Burne, RA ^{[1
]}

机构：

[1] Univ Florida, Dept Oral Biol, Gainesville, FL 32610 USA

来源：

FEMS MICROBIOLOGY LETTERS | 2001年 / 205卷 / 02期

关键词：

biofilm; dental caries; gene regulation; environmental sensing;

D O I：

10.1111/j.1574-6968.2001.tb10952.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Streptococcus mutans has at least six pairs of open reading frames that are homologous to bacterial two-component regulatory systems. Putative response regulators from five out of six of these pairs were successfully mutated by insertion of a kanamycin resistance marker and the effects of inactivation of the genes on the ability of the cells to form biofilms in an in vitro model were assessed. Disruption of the response regulators of four systems had no effect on biofilm formation, whereas disruption of one response regulator caused a substantial decrease in biofilm formation as compared to the wild-type S. mutans. (C) 2001 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：225 / 230

页数：6

共 18 条

[1] Signal transduction via the multi-step phosphorelay: Not necessarily a road less traveled
Appleby, JL
Parkinson, JS
Bourret, RB
[J]. CELL, 1996, 86 (06) : 845 - 848
[2] Burne R A, 1997, Adv Dent Res, V11, P100
[3] Cariogenicity of Streptococcus mutans strains with defects in fructan metabolism assessed in a program-fed specific-pathogen-free rat model
Burne, RA
Chen, YYM
Wexler, DL
Kuramitsu, H
Bowen, WH
[J]. JOURNAL OF DENTAL RESEARCH, 1996, 75 (08) : 1572 - 1577
[4] Bacterial biofilms: A common cause of persistent infections
Costerton, JW
Stewart, PS
Greenberg, EP
[J]. SCIENCE, 1999, 284 (5418) : 1318 - 1322
[5] Fabret C, 1998, J BACTERIOL, V180, P6375
[6] Two-component signal transduction in Bacillus subtilis:: How one organism sees its world
Fabret, C
Feher, VA
Hoch, JA
[J]. JOURNAL OF BACTERIOLOGY, 1999, 181 (07) : 1975 - 1983
[7] Streptococcus parasanguis fimbria-associated adhesin Fap1 is required for biofilm formation
Froeliger, EH
Fives-Taylor, P
[J]. INFECTION AND IMMUNITY, 2001, 69 (04) : 2512 - 2519
[8] A 2-COMPONENT SIGNAL-TRANSDUCING SYSTEM IS INVOLVED IN COMPETENCE AND PENICILLIN SUSCEPTIBILITY IN LABORATORY MUTANTS OF STREPTOCOCCUS-PNEUMONIAE
GUENZI, E
GASC, AM
SICARD, MA
HAKENBECK, R
[J]. MOLECULAR MICROBIOLOGY, 1994, 12 (03) : 505 - 515
[9] CLONING OF THE GENE ENCODING STREPTOCOCCIN A-FF22, A NOVEL LANTIBIOTIC PRODUCED BY STREPTOCOCCUS-PYOGENES, AND DETERMINATION OF ITS NUCLEOTIDE-SEQUENCE
HYNES, WL
FERRETTI, JJ
TAGG, JR
[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1993, 59 (06) : 1969 - 1971
[10] Microbiology - One for all and all for one
Kolter, R
Losick, R
[J]. SCIENCE, 1998, 280 (5361) : 226 - 227

← 1 2 →