Comparison of the redox forms of nitrogen monoxide with the nitrergic transmitter in the rat anococcygeus muscle

被引:29
作者
Li, CG [1 ]
Karagiannis, J [1 ]
Rand, MJ [1 ]
机构
[1] Royal Melbourne Inst Technol, Dept Med Lab Sci, Pharmacol Res Unit, Melbourne, Vic 3001, Australia
关键词
Angeli's salt; carboxy-PTIO; hydroxocobalamin; nitrergic transmitter; nitric oxide; nitrosonium cation; nitrosonium tetrafluoroborate; nitroxyl anion; pyrogallol;
D O I
10.1038/sj.bjp.0702540
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
1 A sustained tone was produced in rat isolated anococcygeus muscles with guanethidine and clonidine and relaxant responses were elicited by electrical stimulation of its nitrergic nerves and by the three redox forms of nitrogen monoxide. 2 The nitroxyl anion (NO-) was donated by dissociation of Angeli's salt; the free radical (NO.) was from an aqueous solution of nitric oxide gas; the nitrosonium cation (NO+) was donated by dissociation of nitrosonium tetrafluoroborate. 3 The concentrations producing approximately 50% relaxations of the anococcygeus muscle were 0.3 mu M for Angeli's salt (nitroxyl), 0.5 mu M for NO. and 100 mu M for nitrosonium tetrafluoroborate. Nitrergic nerve stimulation at 1 Hz for 10 s produced equivalent relaxant responses. 4 The superoxide generator pyrogallol (100 mu M) had no effect on responses to nitrergic nerve stimulation or Angeli's salt but significantly reduced responses to NO. and nitrosonium tetrafluoroborate. 5 The NO. scavenger carboxy-PTIO (100 mu M) had no effect on responses to nitrergic nerve stimulation or Angeli's salt but significantly reduced responses to NO. and nitrosonium tetrafluoroborate. 6 Hydroxocobalamin (30 mu M) had no significant effect on responses to the nitrergic transmitter, enhanced the response to Angeli's salt, and significantly reduced responses to NO. and nitrosonium tetrafluoroborate. 7 The findings suggest that the nitroxyl anion donated by Angeli's salt is a better candidate than NO. to serve as the nitrergic transmitter in the rat anococcygeus muscle, although it still does not behave exactly like the transmitter.
引用
收藏
页码:826 / 834
页数:9
相关论文
共 49 条
[41]   DISCRIMINATION BY THE NO-TRAPPING AGENT, CARBOXY-PTIO, BETWEEN NO AND THE NITRERGIC TRANSMITTER BUT NOT BETWEEN NO AND EDRF [J].
RAND, MJ ;
LI, CG .
BRITISH JOURNAL OF PHARMACOLOGY, 1995, 116 (02) :1906-1910
[42]  
RAND MJ, 1995, NITRIC OXIDE NERVOUS, P228
[43]  
ROCHELLE LG, 1995, J PHARMACOL EXP THER, V275, P48
[44]  
RUBYANI GM, 1986, AM J PHYSIOL, V250, pH822
[45]   No center dot NO from NO synthase [J].
Schmidt, HHHW ;
Hofmann, H ;
Schindler, U ;
Shutenko, ZS ;
Cunningham, DD ;
Feelisch, M .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (25) :14492-14497
[46]   BIOCHEMISTRY OF NITRIC-OXIDE AND ITS REDOX-ACTIVATED FORMS [J].
STAMLER, JS ;
SINGEL, DJ ;
LOSCALZO, J .
SCIENCE, 1992, 258 (5090) :1898-1902
[47]   VASODILATOR EFFECT OF CARBOXY-2-PHENYL-4,4,5,5-TETRAMETHYLIMIDAZOLINE-1-OXYL IN THE CORONARY CIRCULATION - IN-VIVO AND IN-VITRO STUDIES [J].
TSUNODA, R ;
OKUMURA, K ;
ISHIZAKA, H ;
MATSUNAGA, T ;
TABUCHI, T ;
YASUE, H ;
AKAIKE, T ;
SATO, K ;
MAEDA, H .
EUROPEAN JOURNAL OF PHARMACOLOGY, 1994, 262 (1-2) :55-63
[48]   Direct measurement of nitric oxide generation from nitric oxide synthase [J].
Xia, Y ;
Zweier, JL .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (23) :12705-12710
[49]   OXIDATIVE RELEASE OF NITRIC-OXIDE ACCOUNTS FOR GUANYLYL CYCLASE STIMULATING, VASODILATOR AND ANTIPLATETLET ACTIVITY OF PILOTYS ACID - A COMPARISON WITH ANGELIS SALT [J].
ZAMORA, R ;
GRZESIOK, A ;
WEBER, H ;
FEELISCH, M .
BIOCHEMICAL JOURNAL, 1995, 312 :333-339