Detection of secretion from pancreatic islets using chemically modified electrodes

被引：27

作者：

Maghasi, AT

Halsall, HB

Heineman, WR

Rilo, HLR

机构：

[1] Univ Cincinnati, Dept Chem, Cincinnati, OH 45221 USA

[2] Univ Cincinnati, Dept Surg, Ctr Cellular Transplantat, Cincinnati, OH 45267 USA

来源：

ANALYTICAL BIOCHEMISTRY | 2004年 / 326卷 / 02期

关键词：

anodic stripping voltammetry; zinc; bismuth electrode; pancreatic islets;

D O I：

10.1016/j.ab.2003.12.013

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Secretion of insulin from pancreatic islets was monitored indirectly by detecting zinc. Anodic stripping voltammetric measurements of zinc were done on a bismuth-modified electrode. Comparison of the performance of bismuth-modified electrodes and mercury film electrodes showed that bismuth is an appropriate alternative for Zn detection. The bismuth-coated electrode was used to detect zinc in insulin samples and insulin secreted from pancreatic islets upon stimulation with high concentrations of K+, Detection of zinc released from pancreatic islets was done in the culture medium without any further cleanup. This detection method can be used to monitor secretion from pancreatic islets in their native environment. (C) 2004 Elsevier Inc. All rights reserved.

引用

页码：183 / 189

页数：7

共 27 条

[1] Comparison of amperometric methods for detection of exocytosis from single pancreatic β-cells of different species [J].