VEGF-Mediated Proliferation of Human Adipose Tissue-Derived Stem Cells

被引：33

作者：

Chen, Guangfeng ^{[1
]}

Shi, Xiujuan ^{[1
]}

Sun, Chen ^{[1
]}

Li, Min ^{[2
]}

Zhou, Qing ^{[2
]}

Zhang, Chen ^{[1
]}

Huang, Jun ^{[1
]}

Qiu, Yu ^{[1
]}

Wen, Xiangyi ^{[1
]}

Zhang, Yan ^{[1
]}

Zhang, Yushan ^{[1
]}

Yang, Shuzhang ^{[1
]}

Lu, Lixia ^{[1
]}

Zhang, Jieping ^{[1
]}

Yuan, Qionglan ^{[1
]}

Lu, Jianwei ^{[1
]}

Xu, Guotong ^{[1
]}

Xue, Yunyun ^{[1
]}

Jin, Zibing ^{[3
,4
]}

Jiang, Cizhong ^{[2
]}

Ying, Ming ^{[1
,5
]}

Liu, Xiaoqing ^{[1
,5
]}

机构：

[1] Tongji Univ, Sch Med, Peoples Hosp 10, Shanghai 200092, Peoples R China

[2] Tongji Univ, Sch Life Sci & Technol, Shanghai Key Lab Signaling & Dis Res, Shanghai 200092, Peoples R China

[3] Wenzhou Med Coll, Hosp Eye, Div Ophthalm Genet, Wenzhou, Peoples R China

[4] Wenzhou Med Coll, Sch Ophthalmol & Optometry, Lab Stem Cell & Retinal Regenerat, Wenzhou, Peoples R China

[5] Shenzhen Univ, Coll Life Sci, Shenzhen Key Lab Marine Bioresources & Ecol, Shenzhen, Peoples R China

来源：

PLOS ONE | 2013年 / 8卷 / 10期

基金：

中国国家自然科学基金;

关键词：

HUMAN BONE-MARROW; MICRORNA BIOGENESIS;

D O I：

10.1371/journal.pone.0073673

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

070301 [无机化学]; 070403 [天体物理学]; 070507 [自然资源与国土空间规划学]; 090105 [作物生产系统与生态工程];

摘要：

Human adipose tissue-derived stem cells (ADSCs) are an attractive multipotent stem cell source with therapeutic applicability across diverse fields for the repair and regeneration of acute and chronically damaged tissues. In recent years, there has been increasing interest in ADSC for tissue engineering applications. However, the mechanisms underlying the regulation of ADSC proliferation are not fully understood. Here we show that 47 transcripts are up-regulated while 23 are down-regulated in ADSC compared to terminally differentiated cells based on global mRNA profiling and microRNA profiling. Among the up-regulated genes, the expression of vascular endothelial growth factor (VEGF) is fine-tuned by miR-199a-5p. Further investigation indicates that VEGF accelerates ADSC proliferation whereas the multipotency of ADSC remains stable in terms of adipogenic, chondrogenic and osteogenic potentials after VEGF treatment, suggesting that VEGF may serve as an excellent supplement for accelerating ADSC proliferation during in vitro expansion.

引用

页数：10

共 33 条

[1]

Yield of human adipose-derived adult stem cells from liposuction aspirates [J].