Antiviral Effects of the Culture Filtrate from Serratia marcescens Gsm01, against Cucumber mosaic virus (CMV)

被引:5
作者
Thapa, Shree Prasad [2 ]
Lee, Hye Jin [1 ]
Park, Duck Hwan [3 ]
Kim, Sam-Kyu [2 ]
Cho, Jun Mo [2 ]
Cho, Saeyoull [1 ]
Hur, Jang Hyun [2 ]
Lim, Chun Keun [1 ]
机构
[1] Kangwon Natl Univ, Div Bioresources Technol, Chunchon 200701, South Korea
[2] Kangwon Natl Univ, Dept Environm Biol, Chunchon 200701, South Korea
[3] Kangwon Natl Univ, Dept Forest Resources Protect, Chunchon 200701, South Korea
关键词
culture filtrate; Cucumber mosaic virus; Serratia marcescens; GROWTH-PROMOTING RHIZOBACTERIA; TOBACCO NECROSIS VIRUS; SYSTEMIC RESISTANCE; COLLETOTRICHUM-LAGENARIUM; INDUCTION; PLANTS; PROTECTION; TOMATO; RIBONUCLEASES; LOCUS;
D O I
10.5423/PPJ.2009.25.4.369
中图分类号
S [农业科学];
学科分类号
09 ;
摘要
The potential antiviral effects of the culture filtrates (CF) from Serratia marcescens strain Gsm01 against yellow strain of Cucumber mosaic virus (CMV-Y) were investigated. The culture filtrate of S. marcescens strain Gsm01 applied on Chenopodium amaranticolor showed high inhibitory activity, likewise no necrosis appeared when applied on the tobacco plants 2 days before CMV-Y inoculation. When plants were challenge inoculated with CMV-Y for eighteen days, the disease incidence in plants with culture filtrate of S. marcescens Gsm01 did not exceed 59%, whereas 100% of control plants were severely infected. The results of double antibody sandwich-enzyme linked immunosorbent assay (DAS-ELISA), reverse transcriptase polymerase chain reaction (RTPCR), dot blotting, and western blotting showed that culture filtrate treatment highly affected the accumulation of CMV-Y or its CP protein gene in the treated plant leaves. It was also observed that the culture filtrate had no RNase activity on genomic RNAs of CMV-Y, suggesting that culture filtrate may not contain ribosome inactivating proteins (RIPs) or proteins with R-Nase activity. These data shows that culture filtrate of S. marcescens strain Gsm01 seems to be a promising source of antiviral substance for the practical use.
引用
收藏
页码:369 / 375
页数:7
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