A model of the replication fork blocking protein Fob1p based on the catalytic core domain of retroviral integrases

被引：11

作者：

Dlakic, M ^{[1
]}

机构：

[1] Univ Michigan, Sch Med, Dept Biol Chem, Ann Arbor, MI 48109 USA

来源：

PROTEIN SCIENCE | 2002年 / 11卷 / 05期

关键词：

Fob1p; HOT1; rDNA maintenance; DNA recombination; aging in yeast;

D O I：

10.1110/ps.4470102

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The replication fork blocks are common in both prokaryotes and eukaryotes. In most cases, these blocks are associated with increased levels of mitotic recombination. One of the best-characterized replication fork blocks in eukaryotes is found in ribosomal DNA (rDNA) repeats of Saccharomyces cerevisiae. It has been shown that the replication fork blocking protein Fob1p regulates the recombination rate and the number of rDNA copies in S. cerevisiae, but the mechanistic aspects of these events are still poorly understood. Sequence profile searches revealed that Fob1p is related to retroviral integrases. Subsequently, the catalytic domain of HIV-1 integrase was used as a template to build a reliable three-dimensional model of Fob1p. Structural insights from this study may be useful in explaining Fob1p-mediated formation of extrachromosomal rDNA circles that accelerate aging in yeast and recombination events that lead to expansion or contraction of rDNA.

引用

页码：1274 / 1277

页数：4

共 32 条

[1] Gapped BLAST and PSI-BLAST: a new generation of protein database search programs [J].

Altschul, SF ;

Madden, TL ;

Schaffer, AA ;

Zhang, JH ;

Zhang, Z ;

Miller, W ;

Lipman, DJ .

NUCLEIC ACIDS RESEARCH, 1997, 25 (17) :3389-3402

[2] Retroviral integrase, putting the pieces together [J].

Andrake, MD ;

Skalka, AM .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (33) :19633-19636

[3] Holliday junction resolvases and related nucleases: identification of new families, phyletic distribution and evolutionary trajectories [J].

Aravind, L ;

Makarova, KS ;

Koonin, EV .

NUCLEIC ACIDS RESEARCH, 2000, 28 (18) :3417-3432

[4] ATOMIC-STRUCTURE OF THE RUVC RESOLVASE - A HOLLIDAY JUNCTION-SPECIFIC ENDONUCLEASE FROM ESCHERICHIA-COLI [J].

ARIYOSHI, M ;

VASSYLYEV, DG ;

IWASAKI, H ;

NAKAMURA, H ;

SHINAGAWA, H ;

MORIKAWA, K .

CELL, 1994, 78 (06) :1063-1072

[5] IDENTIFICATION OF RESIDUES IN THE MU-TRANSPOSASE ESSENTIAL FOR CATALYSIS [J].

BAKER, TA ;

LUO, L .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (14) :6654-6658

[6]

Bateman A, 2004, NUCLEIC ACIDS RES, V32, pD138, DOI [10.1093/nar/gkp985, 10.1093/nar/gkh121, 10.1093/nar/gkr1065]

[7] THE ARREST OF REPLICATION FORKS IN THE RDNA OF YEAST OCCURS INDEPENDENTLY OF TRANSCRIPTION [J].

BREWER, BJ ;

LOCKSHON, D ;

FANGMAN, WL .

CELL, 1992, 71 (02) :267-276

[8] A REPLICATION FORK BARRIER AT THE 3' END OF YEAST RIBOSOMAL-RNA GENES [J].

BREWER, BJ ;

FANGMAN, WL .

CELL, 1988, 55 (04) :637-643

[9] Branch migration and Holliday junction resolution catalyzed by activities from mammalian cells [J].

Constantinou, A ;

Davies, AA ;

West, SC .

CELL, 2001, 104 (02) :259-268

[10] HIV integrase, a brief overview from chemistry to therapeutics [J].

Craigie, R .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (26) :23213-23216

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