NADPH-dependent thioredoxin reductase and 2-Cys peroxiredoxins are needed for the protection of Mg-protoporphyrin monomethyl ester cyclase

被引：78

作者：

Stenbaek, Anne ^{[1
]}

Hansson, Andreas ^{[2
]}

Wulff, Ragna Peterson ^{[3
]}

Hansson, Mats ^{[3
]}

Dietz, Karl-Josef ^{[4
]}

Jensen, Poul Erik ^{[1
]}

机构：

[1] Univ Copenhagen, Fac Life Sci, Dept Plant Biol & Biotechnol, VKR Res Ctr Proactive Plants, DK-1871 Frederiksberg C, Denmark

[2] Umea Univ, Dept Plant Physiol, Umea Plant Sci Ctr, S-90187 Umea, Sweden

[3] Lund Univ, Dept Biochem, SE-22100 Lund, Sweden

[4] Univ Bielefeld, Dept Plant Physiol & Biochem W5, D-33501 Bielefeld, Germany

来源：

FEBS LETTERS | 2008年 / 582卷 / 18期

关键词：

aerobic cyclase; biosynthetic pathway; chloroplast biogenesis; gene co-expression; hydrogen peroxide scavenging; Arabidopsis thaliana;

D O I：

10.1016/j.febslet.2008.07.006

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The chloroplast-localized NADPH-dependent thioredoxin reductase ( NTRC) has been found to be able to reduce hydrogen peroxide scavenging 2-Cys peroxiredoxins. We show that the Arabidopsis ntrc mutant is perturbed in chlorophyll biosynthesis and accumulate intermediates preceding protochlorophyllide formation. A specific involvement of NTRC during biosynthesis of protochlorophyllide is indicated from in vitro aerobic cyclase assays in which the conversion of Mg-protoporhyrin monomethyl ester into protochlorophyllide is stimulated by addition of the NTRC/2-Cys peroxiredoxin system. These findings support the hypothesis that this NADPH-dependent hydrogen peroxide scavenging system is particularly important during periods with limited reducing power from photosynthesis, e. g. under chloroplast biogenesis. (c) 2008 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：2773 / 2778

页数：6

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