Effect of keigai-rengyo-to, a Japanese Kampo medicine, on neutrophil functions: A possible mechanism of action of keigai-rengyo-to in acne

被引:7
作者
Akamatsu, H
Asada, Y
Horio, T
机构
[1] Department of Dermatology, Kansai Medical University, Osaka
[2] Department of Dermatology, Kansai Medical University, Osaka 570, 10-15 Fumizono-cho, Moriguchi
关键词
keigai-rengyo-to; acne; neutrophil; reactive oxygen species;
D O I
10.1177/030006059702500503
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
On the basis of recent reports that keigai-rengyo-to (TJ-50), an oral Japanese Kampo (herb) medicine, is clinically effective in treating acne, and that tetracyclines are effective against acne by acting directly as an antioxidant on infiltrated neutrophils, we investigated the effect of TJ-50 on the generation of reactive oxygen species (ROS), using human neutrophils and a cell-free, xanthine-xanthine oxidase system. The species investigated were superoxide radical anion (O-2(-)), hydrogen peroxide (H2O2), and hydroxyl radical (OH.). In addition, neutrophil chemotaxis, phagocytosis and calcium concentration, [Ca2+]i in neutrophils were also assessed. TJ-50 significantly decreased neutrophil-generated O-2(-), H2O2 and OH. in a dose-dependent manner. Three kinds of ROS generated in the cell-free system were also reduced in the presence of TJ-50. On the other hand, the medicine did not markedly affect neutrophil chemotaxis, phagocytosis or [Ca2+]i in neutrophils. Our results indicate that the clinical effectiveness of TJ-50 in the treatment of acne may be due partly to its antioxidant action on infiltrated neutrophils.
引用
收藏
页码:255 / 265
页数:11
相关论文
共 32 条
[11]  
Higaki S., 1988, ACTA DERMATOL KYOTO, V83, P537
[12]   PHOSPHOLIPID METHYLATION AND BIOLOGICAL SIGNAL TRANSMISSION [J].
HIRATA, F ;
AXELROD, J .
SCIENCE, 1980, 209 (4461) :1082-1090
[13]   STIMULATION OF PHOSPHOLIPID METHYLATION, CA2+ INFLUX, AND HISTAMINE-RELEASE BY BRIDGING OF IGE RECEPTORS ON RAT MAST-CELLS [J].
ISHIZAKA, T ;
HIRATA, F ;
ISHIZAKA, K ;
AXELROD, J .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1980, 77 (04) :1903-1906
[14]   METABOLISM OF VASOACTIVE PEPTIDES BY HUMAN ENDOTHELIAL CELLS IN CULTURE - ANGIOTENSIN-1 CONVERTING ENZYME (KININASE-2) AND ANGIOTENSINASE [J].
JOHNSON, AR ;
ERDOS, EG .
JOURNAL OF CLINICAL INVESTIGATION, 1977, 59 (04) :684-695
[15]  
JOHNSON PC, 1985, J BIOL CHEM, V260, P2069
[16]   ELABORATION OF TOXIC OXYGEN BY-PRODUCTS BY NEUTROPHILS IN A MODEL OF IMMUNE-COMPLEX DISEASE [J].
JOHNSTON, RB ;
LEHMEYER, JE .
JOURNAL OF CLINICAL INVESTIGATION, 1976, 57 (04) :836-841
[17]   ETHYLENE FORMATION BY POLYMORPHONUCLEAR LEUKOCYTES - ROLE OF MYELOPEROXIDASE [J].
KLEBANOFF, SJ ;
ROSEN, H .
JOURNAL OF EXPERIMENTAL MEDICINE, 1978, 148 (02) :490-506
[18]   EFFECTS OF TETRACYCLINE ON LEUKOTAXIS [J].
MARTIN, RR ;
WARR, GA ;
COUCH, RB ;
YEAGER, H ;
KNIGHT, V .
JOURNAL OF INFECTIOUS DISEASES, 1974, 129 (02) :110-115
[20]   BIOLOGY AND PATHOLOGY OF OXYGEN RADICALS [J].
MCCORD, JM ;
FRIDOVICH, I .
ANNALS OF INTERNAL MEDICINE, 1978, 89 (01) :122-127