Real-time monitoring of yeast cell division by dielectric spectroscopy

被引:94
作者
Asami, K [1 ]
Gheorghiu, E
Yonezawa, T
机构
[1] Kyoto Univ, Inst Chem Res, Kyoto 6110011, Japan
[2] UNESCO, Ctr Biodynam, Natl Inst Biotechnol, Bucharest 77102 1, Romania
[3] Prod Div 1, Osaka 6180001, Japan
关键词
D O I
10.1016/S0006-3495(99)77487-4
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
To assay cell cycle progression in synchronized culture of yeast we have applied dielectric spectroscopy to its real-time monitoring. The dielectric monitoring is based on the electromagnetic induction method, regarded as a nonelectrode method, which has resolved the problems encountered in measurements with metal electrodes, namely electrode polarization and bubble formation on electrodes. In the synchronized culture with temperature-sensitive cell division cycle mutants, the permittivity of the culture broth showed cyclic changes at frequencies below 300 kHz. The increase and decrease in the cyclic changes of the relative permittivity correspond to the increase in cell length and bud size and to the septum formation between mother and daughter cells, respectively.
引用
收藏
页码:3345 / 3348
页数:4
相关论文
共 15 条
[1]  
[Anonymous], [No title captured]
[2]   Dielectric behavior of non-spherical cells in culture [J].
Asami, K ;
Yonezawa, T .
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, 1995, 1245 (03) :317-324
[3]  
Asami K, 1998, HANDBOOK ON ULTRASONIC AND DIELECTRIC CHARACTERIZATION TECHNIQUES FOR SUSPENDED PARTICULATES, P333
[4]   Dielectric behavior of budding yeast in cell separation [J].
Asami, K ;
Gheorghiu, E ;
Yonezawa, T .
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, 1998, 1381 (02) :234-240
[5]   DIELECTRIC ANALYSIS OF YEAST-CELL GROWTH [J].
ASAMI, K ;
YONEZAWA, T .
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, 1995, 1245 (01) :99-105
[6]   Dispersion and absorption in dielectrics I. Alternating current characteristics [J].
Cole, KS ;
Cole, RH .
JOURNAL OF CHEMICAL PHYSICS, 1941, 9 (04) :341-351
[7]   Monitoring cell cycle by impedance spectroscopy: experimental and theoretical aspects [J].
Gheorghiu, E ;
Asami, K .
BIOELECTROCHEMISTRY AND BIOENERGETICS, 1998, 45 (02) :139-143
[8]   THE METHOD OF MOMENTS IN ELECTROMAGNETICS [J].
HARRINGTON, RF .
JOURNAL OF ELECTROMAGNETIC WAVES AND APPLICATIONS, 1987, 1 (03) :181-200
[9]  
HASS SB, 1997, METHOD ENZYMOL, V283, P322
[10]   ONLINE MONITORING OF CELL CONCENTRATIONS BY DIELECTRIC MEASUREMENTS [J].
MISHIMA, K ;
MIMURA, A ;
TAKAHARA, Y ;
ASAMI, K ;
HANAI, T .
JOURNAL OF FERMENTATION AND BIOENGINEERING, 1991, 72 (04) :291-295