Vibrio cholerae O1 outbreak isolates in Mozambique and South Africa in 1998 are multiple-drug resistant, contain the SXT element and the aadA2 gene located on class 1 integrons

被引:84
作者
Dalsgaard, A
Forslund, A
Sandvang, D
Arntzen, L
Keddy, K
机构
[1] Royal Vet & Agr Univ, Dept Vet Microbiol, DK-1870 Frederiksberg C, Denmark
[2] S African Inst Med Res, Div Epidem Enter Dis, ZA-2000 Johannesburg, South Africa
关键词
D O I
10.1093/jac/48.6.827
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
The characteristics of Vibrio cholerae O1 biotype El Tor, serotype Ogawa isolates from outbreaks of cholera in 1998 amongst migrant workers in the South African provinces of Gauteng and Mpumalanga, on the border of Mozambique, are reported. The isolates seem to have originated from the same clone since they are of two closely related BglI ribotypes. These ribotypes had a high similarity to ribotypes of V. cholerae O1 recently found in three South-east Asian countries. Isolates were resistant to furazolidone, streptomycin, sulfamethoxazole, trimethoprim and tetracycline. Only two isolates contained plasmids of 54 and 63 kb in size. PCR and DNA sequencing revealed that the chromosomally located resistance determinants present included an aadA2 gene cassette contained in a class 1 integron; the SXT element, which is a transposon-like element containing resistance genes; and the tetA gene. A co-transfer of chromosomal closely located genes encoding the SXT element and tetA was shown by mating experiments, PCR and pulsed-field gel electrophoresis analyses. Our study shows for the first time that multiple-resistant V. cholerae O1 isolates containing class 1 integrons and the SXT element were responsible for cholera outbreaks in Southern Africa. Studies are needed to determine the spread of this multiple-resistant O1 strain and further genetic details of the association of the SXT element, tetA and class 1 integrons, including their means of transfer.
引用
收藏
页码:827 / 838
页数:12
相关论文
共 69 条
[1]   SEQUENCE OF A CLASS-E TETRACYCLINE RESISTANCE GENE FROM ESCHERICHIA-COLI AND COMPARISON OF RELATED TETRACYCLINE EFFLUX PROTEINS [J].
ALLARD, JD ;
BERTRAND, KP .
JOURNAL OF BACTERIOLOGY, 1993, 175 (14) :4554-4560
[2]   NUCLEOTIDE-SEQUENCE OF CLASS-D TETRACYCLINE RESISTANCE GENES FROM SALMONELLA-ORDONEZ [J].
ALLARD, JD ;
GIBSON, ML ;
VU, LH ;
NGUYEN, TT ;
BERTRAND, KP .
MOLECULAR & GENERAL GENETICS, 1993, 237 (1-2) :301-305
[3]  
ALTSCHUL SF, 1990, J MOL BIOL, V215, P403, DOI 10.1006/jmbi.1990.9999
[4]   DISTRIBUTION OF TETRACYCLINE RESISTANCE DETERMINANTS AMONG GRAM-NEGATIVE BACTERIA ISOLATED FROM POLLUTED AND UNPOLLUTED MARINE-SEDIMENTS [J].
ANDERSEN, SR ;
SANDAA, RA .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1994, 60 (03) :908-912
[5]  
Athan E, 1998, S AFR MED J, V88, P1306
[6]   Rapid spread of the new clone of Vibrio cholerae O1 biotype El Tor in cholera endemic areas in India [J].
Bag, PK ;
Maiti, S ;
Sharma, C ;
Ghosh, A ;
Basu, A ;
Mitra, R ;
Bhattacharya, SK ;
Nakamura, S ;
Yamasaki, S ;
Takeda, Y ;
Nair, GB .
EPIDEMIOLOGY AND INFECTION, 1998, 121 (02) :245-251
[7]   Characterisation of recently emerged multiple antibiotic-resistant Salmonella enterica serovar typhimurium DT104 and other multiresistant phage types from Danish pig herds [J].
Baggesen, DL ;
Aarestrup, FM .
VETERINARY RECORD, 1998, 143 (04) :95-97
[8]  
Bej A K, 1992, PCR Methods Appl, V1, P151
[9]   COMPLETE SEQUENCE OF PSC101 [J].
BERNARDI, A ;
BERNARDI, F .
NUCLEIC ACIDS RESEARCH, 1984, 12 (24) :9415-9426
[10]   CHARACTERIZATION OF IN0 OF PSEUDOMONAS-AERUGINOSA PLASMID PVS1, AN ANCESTOR OF INTEGRONS OF MULTIRESISTANCE PLASMIDS AND TRANSPOSONS OF GRAM-NEGATIVE BACTERIA [J].
BISSONNETTE, L ;
ROY, PH .
JOURNAL OF BACTERIOLOGY, 1992, 174 (04) :1248-1257