Drug response assay system in a microchip using human hepatoma cells

被引：14

作者：

Tanaka, Y

Sato, K

Yamato, M

Okano, T

Kitamori, T

机构：

[1] Univ Tokyo, Sch Engn, Dept Appl Chem, Bunkyo Ku, Tokyo 1138656, Japan

[2] CREST, Japan Sci & Technol Agcy, Kawaguchi, Saitama 3320012, Japan

[3] Univ Tokyo, Grad Sch Agr & Life Sci, Dept Appl Biol Chem, Tokyo 1138657, Japan

[4] Kanagawa Acad Sci & Technol, Micro Chem Grp, Special Res Lab Opt Sci, Kawasaki, Kanagawa 2130012, Japan

[5] Tokyo Womens Med Univ, Inst Adv Biomed Engn & Sci, Tokyo 1628666, Japan

来源：

ANALYTICAL SCIENCES | 2004年 / 20卷 / 03期

关键词：

D O I：

10.2116/analsci.20.411

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A microchip-based cell response assay system to an anticancer agent was developed. The hepatoma cell line HepG2 was used to assess the effects of an anticancer agent, doxorubicin. The required cell number was reduced by two orders, and the observation of the time course of cell response became possible. The system clearly showed that treatment with higher doses of the drug or longer exposure times gave more effects to cells. The possibilities of novel drug response studies or toxicity assay system were demonstrated.

引用

页码：411 / 413

页数：3

共 17 条

[1]

FURUKAWA T, 1995, CLIN CANCER RES, V1, P305

[2] On-chip integration of neutral ionophore-based ion pair extraction reaction [J].