Genes directly controlled by CtrA, a master regulator of the Caulobacter cell cycle

被引:305
作者
Laub, MT [1 ]
Chen, SL [1 ]
Shapiro, L [1 ]
McAdams, HH [1 ]
机构
[1] Stanford Univ, Sch Med, Beckman Ctr, Dept Dev Biol, Palo Alto, CA 94304 USA
关键词
D O I
10.1073/pnas.062065699
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Studies of the genetic network that controls the Caulobacter cell cycle have identified a response regulator, CtrA, that controls, directly or indirectly, one-quarter of the 553 cell cycle-regulated genes. We have performed in vivo genomic binding site analysis of the CtrA protein to identify which of these genes have regulatory regions bound directly by CtrA. By combining these data with previous global analysis of cell cycle transcription patterns and gene expression profiles of mutant ctrA strains, we have determined that CtrA directly regulates at least 95 genes. The total group of CtrA-regulated genes includes those involved in polar morphogenesis, DNA replication initiation, DNA methylation, cell division, and cell wall metabolism. Also among the genes in this notably large regulon are 14 that encode regulatory proteins, including 10 two-component signal transduction regulatory proteins. Identification of additional regulatory genes activated by CtrA will serve to directly connect new regulatory modules to the network controlling cell cycle progression.
引用
收藏
页码:4632 / 4637
页数:6
相关论文
共 26 条
  • [21] Ordered expression of ftsQA and ftsZ during the Caulobacter crescentus cell cycle
    Sackett, MJ
    Kelly, AJ
    Brun, YV
    [J]. MOLECULAR MICROBIOLOGY, 1998, 28 (03) : 421 - 434
  • [22] Serial regulation of transcriptional regulators in the yeast cell cycle
    Simon, I
    Barnett, J
    Hannett, N
    Harbison, CT
    Rinaldi, NJ
    Volkert, TL
    Wyrick, JJ
    Zeitlinger, J
    Gifford, DK
    Jaakkola, TS
    Young, RA
    [J]. CELL, 2001, 106 (06) : 697 - 708
  • [23] Identification and cell cycle control of a novel pilus system in Caulobacter crescentus
    Skerker, JM
    Shapiro, L
    [J]. EMBO JOURNAL, 2000, 19 (13) : 3223 - 3234
  • [24] Comprehensive identification of cell cycle-regulated genes of the yeast Saccharomyces cerevisiae by microarray hybridization
    Spellman, PT
    Sherlock, G
    Zhang, MQ
    Iyer, VR
    Anders, K
    Eisen, MB
    Brown, PO
    Botstein, D
    Futcher, B
    [J]. MOLECULAR BIOLOGY OF THE CELL, 1998, 9 (12) : 3273 - 3297
  • [25] Proteolysis of the McpA chemoreceptor does not require the Caulobacter major chemotaxis operon
    Tsai, JW
    Alley, MRK
    [J]. JOURNAL OF BACTERIOLOGY, 2000, 182 (02) : 504 - 507
  • [26] FtsI and FtsW are localized to the septum in Escherichia coli
    Wang, L
    Khattar, MK
    Donachie, WD
    Lutkenhaus, J
    [J]. JOURNAL OF BACTERIOLOGY, 1998, 180 (11) : 2810 - 2816